Litvinova L S, Shupletsova V V, Yurova K A, Khaziakhmatova O G, Todosenko N M, Khlusova M Yu, Slepchenko G B, Cherempey E G, Sharkeev Yu P, Komarova E G, Sedelnikova M B, Malashchenko V V, Melashchenko E S, Khlusov I A
Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russia.
Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, 634050, Russia.
Dokl Biochem Biophys. 2017 Sep;476(1):310-315. doi: 10.1134/S1607672917050076. Epub 2017 Nov 4.
The Cell-IQ continuous surveillance system allowed us to establish the following changes in a 14- day culture in vitro: a twofold suppression of the directional migration of multipotent mesenchymal stromal cells of human adipose tissue (MMSC-AT) towards the samples with a microarc calcium phosphate (CP) coating from synthetic hydroxyapatite; a tenfold decrease in the cell mass on the interphase with the samples, which was accompanied by a slight reduction in the expression of membrane determinants of stromal stem cells; and an enhancement of their osteogenic differentiation (osteocalcin secretion and mineralized matrix formation) on the 21st day of the study. Calcium phosphate particles, but not the calcium and phosphorus ions, may trigger the phenotypic transformation of the MMSC-AT behavior in vitro.
Cell-IQ连续监测系统使我们能够确定人脂肪组织多能间充质基质细胞（MMSC-AT）在体外14天培养过程中的以下变化：与合成羟基磷灰石微弧磷酸钙（CP）涂层样本相比，人脂肪组织多能间充质基质细胞向其定向迁移受到两倍抑制；与样本界面处的细胞质量减少了十倍，同时基质干细胞膜决定簇的表达略有降低；在研究的第21天，其成骨分化增强（骨钙素分泌和矿化基质形成）。磷酸钙颗粒而非钙和磷离子可能会触发体外MMSC-AT行为的表型转变。
