Center for Immunology and Cellular Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236000, Russia.
Department of Pathophysiology, Siberian State Medical University, Tomsk 634050, Russia.
Int J Mol Sci. 2020 Oct 16;21(20):7682. doi: 10.3390/ijms21207682.
The manufacture of biomaterial surfaces with desired physical and chemical properties that can directly induce osteogenic differentiation without the need for biochemical additives is an excellent strategy for controlling the behavior of mesenchymal stem cells (MSCs) in vivo. We studied the cellular and molecular reactions of MSCs to samples with a double-sided calcium phosphate (CaP) coating and an average roughness index (Ra) of 2.4-4.6 µm. The study aimed to evaluate the effect of a three-dimensional matrix on the relative mRNA expression levels of genes associated with the differentiation and maturation of MSCs toward osteogenesis (, , , , and ) under conditions of distant interaction in vitro. Correlations were revealed between the mRNA expression of some osteogenic and cytokine/chemokine genes and the secretion of cytokines and chemokines that may potentiate the differentiation of cells into osteoblasts, which indicates the formation of humoral components of the extracellular matrix and the creation of conditions supporting the establishment of hematopoietic niches.
具有理想物理和化学性能的生物材料表面的制造，可以直接诱导成骨分化，而无需生化添加剂，这是控制间充质干细胞（MSCs）在体内行为的绝佳策略。我们研究了具有双面磷酸钙（CaP）涂层和平均粗糙度指数（Ra）为 2.4-4.6 µm 的样品中 MSC 的细胞和分子反应。该研究旨在评估三维基质对与 MSC 向成骨分化相关的基因的相对 mRNA 表达水平的影响（、、、、和），在体外远距离相互作用的条件下。一些成骨和细胞因子/趋化因子基因的 mRNA 表达与细胞分化为成骨细胞的细胞因子和趋化因子的分泌之间存在相关性，这表明细胞外基质的体液成分的形成和支持造血龛建立的条件的创造。
