Kyriakidou Maria, Anastassopoulou Jane, Tsakiris Aristeidis, Koui Maria, Theophanides Theophile
Laboratory of Radiation Chemistry & Biospectroscopy, Chemical Engineering School, National Technical University of Athens, Athens, Greece.
Department of Plastic and Reconstructive Surgery, 401 General Military Hospital of Athens, Athens, Greece.
In Vivo. 2017 Nov-Dec;31(6):1131-1137. doi: 10.21873/invivo.11179.
BACKGROUND/AIM: Mid-infrared spectroscopy (4000-500 cm) was used to analyze the spectral changes and differences of the characteristic absorption bands of the skin components due to cancer development for early clinical diagnosis.
Human biopsies from basal cell carcinoma, malignant melanoma, and nevus were used, while normal skin tissue served as a control.
The high quality of Fourier-transform infrared (FT-IR) spectra showed that upon cancer development the intensity of the absorption band at approximately 3062 cm was increased, indicating that most of the proteins had the configuration of amide B and the β-sheet protein structure predominated. The stretching vibration bands of vCH in the region 2950-2850 cm were increased in melanoma and nevus, while were less pronounced in basal cell carcinoma due to the increased lipophilic environment. In addition, the intensity of a new band at 1744 cm, which is assigned to aldehyde, was increased in melanoma and nevus and appeared as a shoulder in the spectra of normal skin. The absorption band of amide I at 1650 cm was split into two bands, at 1650 cm and 1633 cm, due to the presence of both α-helix and random coil protein conformations for melanoma and nevus. This was confirmed from the amide II band at 1550 cm, which shifted to lower frequencies at 1536 cm and 1540 cm for basal cell carcinoma and melanoma, respectively, indicating a damage of the native structure of proteins. The bands at 841 and 815 cm, which are assigned to B-DNA and Z-DNA, respectively, indicated that only the bands of the cancerous Z-DNA form are pronounced in melanoma, while in BCC both the characteristic bands of B-DNA and Z-DNA forms are found.
It is proposed that the bands described above could be used as "diagnostic marker" bands for DNA forms, in the diagnosis of skin cancer.
背景/目的：利用中红外光谱（4000 - 500厘米）分析因癌症发展导致的皮肤成分特征吸收带的光谱变化及差异，以用于早期临床诊断。
使用来自基底细胞癌、恶性黑色素瘤和痣的人体活检样本，同时以正常皮肤组织作为对照。
傅里叶变换红外（FT - IR）光谱的高质量显示，癌症发展时，约3062厘米处吸收带的强度增加，表明大多数蛋白质具有酰胺B的构型且β - 折叠蛋白质结构占主导。在2950 - 2850厘米区域，vCH的伸缩振动带在黑色素瘤和痣中增加，而在基底细胞癌中由于亲脂环境增加不太明显。此外，在1744厘米处归属于醛的新吸收带强度在黑色素瘤和痣中增加，并在正常皮肤光谱中以肩峰形式出现。由于黑色素瘤和痣中同时存在α - 螺旋和无规卷曲蛋白质构象，1650厘米处的酰胺I吸收带分裂为1650厘米和1633厘米处的两个带。这从1550厘米处的酰胺II带得到证实，对于基底细胞癌和黑色素瘤，该带分别移至较低频率的1536厘米和1540厘米，表明蛋白质天然结构受损。分别归属于B - DNA和Z - DNA的841厘米和815厘米处的带表明，仅癌性Z - DNA形式的带在黑色素瘤中明显，而在基底细胞癌中同时发现B - DNA和Z - DNA形式的特征带。
建议上述吸收带可作为DNA形式的“诊断标志物”带，用于皮肤癌的诊断。
