Department of Advanced Materials and Chemical Engineering, Hannam University, Daejeon 34054, Republic of Korea.
Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 52727, Republic of Korea.
Carbohydr Polym. 2018 Jan 15;180:216-225. doi: 10.1016/j.carbpol.2017.10.029. Epub 2017 Oct 7.
We prepared in situ gelling alginate (ALG)/hyaluronic acid (HA) hydrogels with a controllable gelation rate using CaSO as a crosslinking agent and NaHPO as a crosslinking retardation agent. The ALG/HA hydrogels provided sustained release of bone morphogenetic protein-2 (BMP-2) immobilized in the hydrogels over 5 weeks. The BMP-2-immobilized ALG/HA hydrogels with different ALG/HA ratios were investigated for their in vitro osteogenic differentiation behavior of human bone marrow stem cells (hBMSCs) and in vivo bone regeneration behavior using an animal model (mandibular defect model of miniature pigs). Our findings from cell culture and animal study demonstrated that the osteogenic differentiation of hBMSCs was improved with increasing HA composition in the hydrogel. The hBMSCs/BMP-2-immobilized ALG/HA hydrogel allowed greatly enhanced osteogenic differentiation of hBMSCs (in vitro) and bone regeneration (in vivo) compared with the ALG/HA hydrogel itself and single hBMSCs- or BMP-2-immobilized hydrogel groups.
我们使用 CaSO 作为交联剂和 NaHPO 作为交联延迟剂，制备了具有可控凝胶化速率的原位凝胶化藻酸盐（ALG）/透明质酸（HA）水凝胶。ALG/HA 水凝胶为固定在水凝胶中的骨形态发生蛋白 2（BMP-2）提供了长达 5 周的持续释放。我们研究了不同 ALG/HA 比例的 BMP-2 固定 ALG/HA 水凝胶在体外人骨髓间充质干细胞（hBMSCs）的成骨分化行为和动物模型（小型猪下颌骨缺损模型）中的体内骨再生行为。我们的细胞培养和动物研究结果表明，随着水凝胶中 HA 组成的增加，hBMSCs 的成骨分化得到改善。与 ALG/HA 水凝胶本身以及单独的 hBMSCs 或 BMP-2 固定水凝胶组相比，hBMSCs/BMP-2 固定 ALG/HA 水凝胶显著增强了 hBMSCs 的成骨分化（体外）和骨再生（体内）。
