Kim Eun-Cheol, Yoon Sun Jung, Noh Kwantae, Lee Deok-Won
J Nanosci Nanotechnol. 2017 Jan;17(1):143-52. doi: 10.1166/jnn.2017.12380.
In the present study, we evaluated the potential of poly-l-lysine/hyaluronic acid (HA/PLL) hydrogels containing curcumin (CUR) and bone morphogenetic protein-2 (BMP-2) as bone tissue regeneration scaffolds. Hydrogels HP-1˜2 were formed by amide bonds via the condensation reactions between 0.02 μmol HA and 0.06–0.12 μmol poly-l-lysine · hydrobromide (PLL · HBr). Physical, chemical, and thermal analyses revealed that the amount of PLL · HBr significantly influenced hydrogel properties. Based on an In Vitro MG-63 cell proliferation test, HP-1˜2 were cytocompatible, and all hydrogels containing different amounts of CUR and BMP-2, except for HA0.02/PLL0.06/CUR20/BMP-2100 (HPCB-4), resulted in cell proliferation above 80%. An In Vitro release test showed that CUR and BMP-2 were consistently released from HA0.02/PLL0.06/CUR15 (HPC), HA0.02/PLL0.06/BMP-2100 (HPB), HA0.02/PLL0.06/CUR15/BMP-210 , 50 , or 100 (HPCB-1˜3), and HA0.02/PLL0.06/CUR10 or 20/BMP-2100 (HPCB-4˜5) for 7 and 28 days, respectively. In Vitro ALP activity and calcium deposition and In Vivo micro-computed tomography (micro-CT) tests demonstrated the potential application of HPCB-3 as bone tissue regeneration scaffolds, suggesting that bone tissue regeneration can be optimized by controlling the amounts of CUR and BMP-2.
在本研究中，我们评估了含有姜黄素（CUR）和骨形态发生蛋白-2（BMP-2）的聚-L-赖氨酸/透明质酸（HA/PLL）水凝胶作为骨组织再生支架的潜力。水凝胶HP-1至2通过0.02 μmol HA与0.06 - 0.12 μmol聚-L-赖氨酸·氢溴酸盐（PLL·HBr）之间的缩合反应由酰胺键形成。物理、化学和热分析表明，PLL·HBr的量显著影响水凝胶性能。基于体外MG-63细胞增殖试验，HP-1至2具有细胞相容性，并且除了HA0.02/PLL0.06/CUR20/BMP-2100（HPCB-4）之外，所有含有不同量CUR和BMP-2的水凝胶导致细胞增殖高于80%。体外释放试验表明，CUR和BMP-2分别从HA0.02/PLL0.06/CUR15（HPC）、HA0.02/PLL0.06/BMP-2100（HPB）、HA0.02/PLL0.06/CUR15/BMP-210、50或100（HPCB-1至3）以及HA0.02/PLL0.06/CUR10或20/BMP-2100（HPCB-4至5）中持续释放7天和28天。体外碱性磷酸酶活性和钙沉积以及体内微型计算机断层扫描（micro-CT）试验证明了HPCB-3作为骨组织再生支架的潜在应用，表明通过控制CUR和BMP-2的量可以优化骨组织再生。
