Department of Chemistry, Faculty of Art and Sciences, Duzce University, Duzce 81620, Turkey.
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey; Nanobiz R & D Ltd., Gallium Bld. No.18, METU, Science Park, Ankara, Turkey.
Carbohydr Polym. 2018 Jan 15;180:226-230. doi: 10.1016/j.carbpol.2017.10.039. Epub 2017 Oct 10.
A novel chitosan-based ratiometric fluorescent probe incorporating an EDTA-Eu complex as the sensing unit and fluorescein dye as the internal standard was designed to detect dipicolinic acid (DPA) as an anthrax biomarker with high sensitivity and selectivity. The fluorescence intensity of fluorescein dye attached to the chitosan backbone remains constant as an internal reference, while the Eu emission increased linearly upon the consecutive addition of DPA. The selectivity studies were performed by adding different competitive aromatic ligands to the sensing environment and no signifacant fluorescence response was observed. The results demonstrated the superior selectivity of the system to DPA. Overall, this novel chitosan-based ratiometric fluorescent probe enables ratiometric and sensitive DPA detection over nanomolar concentrations (as low as 10nM) and displays straightforward selectivity over other competitive aromatic ligands.
一种新型的基于壳聚糖的比率荧光探针，将 EDTA-Eu 配合物作为传感单元，荧光素染料作为内标，用于高灵敏度和选择性地检测炭疽生物标志物二吡啶羧酸（DPA）。连接在壳聚糖主链上的荧光素染料的荧光强度保持不变作为内参，而 Eu 发射强度在连续加入 DPA 时呈线性增加。通过向传感环境中添加不同的竞争性芳香族配体来进行选择性研究，没有观察到明显的荧光响应。结果表明该体系对 DPA 具有优异的选择性。总的来说，这种新型的基于壳聚糖的比率荧光探针能够在纳摩尔浓度（低至 10nM）下进行比率和灵敏的 DPA 检测，并对其他竞争性芳香族配体表现出直接的选择性。
