Division of Biotechnology, Department of Applied Science, University of Technology, Baghdad, Iraq.
Division of Biotechnology, Department of Applied Science, University of Technology, Baghdad, Iraq.
Microbes Infect. 2018 Feb;20(2):89-100. doi: 10.1016/j.micinf.2017.10.007. Epub 2017 Nov 8.
Autophagy is a cellular process for maintaining cellular homeostasis. This process can be induced by different factors, such as immune stimuli and pathogen-associated molecules. Autophagy has an important role in the control of IL-1β secretion by macrophages and other cell types. In present study, we describe a novel role for Iraqi propolis affecting autophagy in controlling the secretion of IL-1β in bone-marrow macrophages (BMDMs). After infection with Pseudomonas aeruginosa in the presence of propolis, the degradation of IL-1β was induced, and the activity of inflammasome was reduced. Iraqi propolis-induced autophagy in in vitro and in vivo models decreased the levels of IL-1β and caspase-1. Results indicated that IL-1β pathway production is regulated by autophagy via two different novel mechanisms, namely, regulation of the activation of NLRC4 inflammasome and IL-1β targeting for lysosomal degradation.
自噬是一种维持细胞内稳态的细胞过程。这个过程可以被不同的因素诱导，例如免疫刺激和病原体相关分子。自噬在控制巨噬细胞和其他细胞类型中 IL-1β 的分泌方面起着重要作用。在本研究中，我们描述了伊拉克蜂胶在控制骨髓巨噬细胞（BMDM）中 IL-1β 分泌方面的一种新的自噬作用。在存在蜂胶的情况下感染铜绿假单胞菌后，诱导了 IL-1β 的降解，并且炎症小体的活性降低。在体外和体内模型中，伊拉克蜂胶诱导的自噬降低了 IL-1β 和 caspase-1 的水平。结果表明，自噬通过两种不同的新机制调节 IL-1β 途径的产生，即 NLRC4 炎症小体的激活调节和 IL-1β 靶向溶酶体降解。
