Hamerly Timothy, Everett Jake A, Paris Nina, Fisher Steve T, Karunamurthy Arivarasan, James Garth A, Rumbaugh Kendra P, Rhoads Daniel D, Bothner Brian
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA.
Department of Surgery and TTUHSC Surgery Burn Center of Research Excellence, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.
Anal Biochem. 2017 Dec 15;539:144-148. doi: 10.1016/j.ab.2017.10.012. Epub 2017 Oct 28.
Monitoring patients with burn wounds for infection is standard practice because failure to rapidly and specifically identify a pathogen can result in poor clinical outcomes, including death. Therefore, a method that facilitates detection and identification of pathogens in situ within minutes of biopsy would be a significant benefit to clinicians. Mass spectrometry is rapidly becoming a standard tool in clinical settings, capable of identifying specific pathogens from complex samples. Imaging mass spectrometry (IMS) expands the information content by enabling spatial resolution of biomarkers in tissue samples as in histology, without the need for specific stains/antibodies. Herein, a murine model of thermal injury was used to study infection of burn tissue by Pseudomonas aeruginosa. This is the first use of IMS to detect P. aeruginosa infection in situ from thermally injured tissue. Multiple molecular features could be spatially resolved to infected or uninfected tissue. This demonstrates the potential use of IMS in a clinical setting to aid doctors in identifying both presence and species of pathogens in tissue.
监测烧伤患者是否感染是标准做法，因为未能快速、准确地识别病原体可能导致包括死亡在内的不良临床结果。因此，一种能在活检后几分钟内原位促进病原体检测和鉴定的方法将对临床医生大有裨益。质谱分析法正迅速成为临床环境中的标准工具，能够从复杂样本中识别特定病原体。成像质谱法（IMS）通过实现组织样本中生物标志物的空间分辨率，如同组织学那样，无需特定染色/抗体，从而扩展了信息内容。在此，使用热损伤小鼠模型来研究铜绿假单胞菌对烧伤组织的感染。这是首次使用IMS从热损伤组织中原位检测铜绿假单胞菌感染。多个分子特征能够在空间上区分感染组织和未感染组织。这证明了IMS在临床环境中的潜在用途，有助于医生识别组织中病原体的存在及种类。
