Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, Republic of Korea.
Probiotics Antimicrob Proteins. 2021 Jun;13(3):788-795. doi: 10.1007/s12602-020-09740-w. Epub 2021 Jan 17.
Oxidative stress plays an important role in exacerbating neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease, and Huntington's disease. In a previous study, Lactobacillus plantarum 200655 was shown to possess probiotic and antioxidant potential. The current study aimed to evaluate the neuroprotective effects of heat-killed L. plantarum 200655. We incubated intestinal cells (HT-29) with heat-killed L. plantarum 200655 in a conditioned medium (CM) and found that the brain-derived neurotrophic factor (BDNF) mRNA level was elevated in the HT-29 cells and the CM contained high concentrations of BDNF. The CM protected neuroblastoma cells (SH-SY5Y) from hydrogen peroxide (HO)-induced toxicity. Moreover, the CM increased BDNF and tyrosine hydroxylase (TH) mRNA expression and significantly reduced the apoptosis-related Bax/Bcl-2 ratio in HO-treated SH-SY5Y cells. At the protein level, the CM resulted in downregulation of caspase-3. These results indicate that L. plantarum 200655 might be used as a prophylactic functional ingredient to prevent neurodegenerative disease.
氧化应激在加剧神经退行性疾病（如阿尔茨海默病、帕金森病和亨廷顿病）方面发挥着重要作用。在之前的一项研究中，植物乳杆菌 200655 被证明具有益生菌和抗氧化潜力。本研究旨在评估热灭活植物乳杆菌 200655 的神经保护作用。我们将热灭活的植物乳杆菌 200655 与肠细胞（HT-29）在条件培养基（CM）中孵育，发现 HT-29 细胞中的脑源性神经营养因子（BDNF）mRNA 水平升高，并且 CM 中含有高浓度的 BDNF。CM 可保护神经母细胞瘤细胞（SH-SY5Y）免受过氧化氢（HO）诱导的毒性。此外，CM 增加了 BDNF 和酪氨酸羟化酶（TH）mRNA 的表达，并显著降低了 HO 处理的 SH-SY5Y 细胞中的凋亡相关 Bax/Bcl-2 比值。在蛋白质水平上，CM 导致 caspase-3 下调。这些结果表明，植物乳杆菌 200655 可能被用作预防神经退行性疾病的预防性功能性成分。
