Systems Immunity Research Institute, School of Medicine, Cardiff University, Cardiff CF14 4XW, UK.
Cultech Limited, Unit 2 Christchurch Road, Baglan Industrial Park, Port Talbot SA12 7BZ, UK.
Int J Mol Sci. 2023 Feb 28;24(5):4683. doi: 10.3390/ijms24054683.
Aging and metabolic syndrome are associated with neurodegenerative pathologies including Alzheimer's disease (AD) and there is growing interest in the prophylactic potential of probiotic bacteria in this area. In this study, we assessed the neuroprotective potential of the Lab4P probiotic consortium in both age and metabolically challenged 3xTg-AD mice and in human SH-SY5Y cell culture models of neurodegeneration. In mice, supplementation prevented disease-associated deteriorations in novel object recognition, hippocampal neurone spine density (particularly thin spines) and mRNA expression in hippocampal tissue implying an anti-inflammatory impact of the probiotic, more notably in the metabolically challenged setting. In differentiated human SH-SY5Y neurones challenged with β-Amyloid, probiotic metabolites elicited a neuroprotective capability. Taken together, the results highlight Lab4P as a potential neuroprotective agent and provide compelling support for additional studies in animal models of other neurodegenerative conditions and human studies.
衰老和代谢综合征与神经退行性病变有关，包括阿尔茨海默病（AD），人们对益生菌在这方面的预防潜力越来越感兴趣。在这项研究中，我们评估了 Lab4P 益生菌联合体在年龄和代谢挑战的 3xTg-AD 小鼠以及神经退行性变的人类 SH-SY5Y 细胞培养模型中的神经保护潜力。在小鼠中，补充剂预防了新型物体识别、海马神经元棘突密度（特别是细棘突）和海马组织中 mRNA 表达的与疾病相关的恶化，这表明益生菌具有抗炎作用，在代谢挑战的情况下更为明显。在受到β-淀粉样蛋白挑战的分化人类 SH-SY5Y 神经元中，益生菌代谢产物引发了神经保护能力。总之，这些结果强调了 Lab4P 作为一种潜在的神经保护剂的作用，并为其他神经退行性疾病的动物模型和人类研究提供了有力的支持。
