Yu Y, Zhang J, Zhu R, Zhao R, Chen J, Jin J, Tian Y, Su S B
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China.
Eye Institute, Affiliated Hospital of Nantong University, Nantong 226001, China.
Curr Mol Med. 2017 Dec 7;17(4):280-286. doi: 10.2174/1566524017666171106111440.
To explore the expression profile of angiogenic factors associated with proliferative diabetic retinopathy (PDR).
Undiluted vitreous humor samples were obtained from 10 diabetic patients with PDR (10 eyes) and 9 non-diabtic patients (9 eyes). The concentrations of 60 angiogenic factors in the vitreous humor samples were measured by RayBio Angiogenic Cytokine Antibody Array. Some differentially expressed factors were further confirmed in vitreous humor by enzyme-linked immunosorbent assay (ELISA).
Compared with the non-diabetic controls, 20 differentially expressed factors with more than 1.50 fold changes were detected in patients with PDR. The median concentration of vascular endothelial growth factor (VEGF), interleukin 6 (IL-6), angiopoietin (ANG)-1, ANG-2, urokinase-type plasminogen activator receptor (uPAR), Follistatin and matrix metalloproteinases 9 (MMP-9) was significantly increased in vitreous samples from PDR compared to controls (P < 0.05). However, (MCP)-1, Angiogenin and Leptin was significantly lower in PDR eyes compared to controls (P < 0.05). In the verification assay using ELISA, ANG-1, ANG-2, IL-6, VEGF, MMP-9, hepatocyte growth factor (HGF) and placenta growth factor (PIGF) concentration were increased in patients with PDR compared to controls (all P-values < 0.05).
This is the first report of a comprehensive multiplex analysis to identify angiogenic factors associated with PDR. These angiogenic factors may contribute to the pathogenesis of PDR and may be targets for therapeutic strategies of PDR.
探讨与增殖性糖尿病视网膜病变（PDR）相关的血管生成因子的表达谱。
从10例患有PDR的糖尿病患者（10只眼）和9例非糖尿病患者（9只眼）中获取未稀释的玻璃体液样本。通过RayBio血管生成细胞因子抗体阵列检测玻璃体液样本中60种血管生成因子的浓度。一些差异表达因子通过酶联免疫吸附测定（ELISA）在玻璃体液中进一步得到证实。
与非糖尿病对照组相比，在PDR患者中检测到20种差异表达因子，其变化倍数超过1.50倍。与对照组相比，PDR玻璃体样本中血管内皮生长因子（VEGF）、白细胞介素6（IL-6）、血管生成素（ANG）-1、ANG-2、尿激酶型纤溶酶原激活剂受体（uPAR）、卵泡抑素和基质金属蛋白酶9（MMP-9）的中位数浓度显著升高（P<0.05）。然而，与对照组相比，PDR眼中单核细胞趋化蛋白（MCP）-1、血管生成素和瘦素显著降低（P<0.05）。在使用ELISA的验证试验中，与对照组相比，PDR患者中ANG-1、ANG-2、IL-6、VEGF、MMP-9、肝细胞生长因子（HGF）和胎盘生长因子（PIGF）的浓度升高（所有P值<0.05）。
这是首次对与PDR相关的血管生成因子进行全面多重分析的报告。这些血管生成因子可能有助于PDR的发病机制，并且可能是PDR治疗策略的靶点。
