National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan, China.
Joint International Research Laboratory of Food & Medicine Resource Function, Kaifeng, 475004 Henan, China.
Oxid Med Cell Longev. 2020 Mar 12;2020:1603735. doi: 10.1155/2020/1603735. eCollection 2020.
The immunomodulatory effects of flower polysaccharide (MHFP) were investigated in this paper. The model of immunosuppressive mice was established by cyclophosphamide, which was treated with different dosages of MHFP (600, 400, and 200 mg/kg·d). The results showed that MHFP significantly increased the index of the spleen and thymus and improved the atrophy of immune organs. MHFP enhanced the ability of carbon clearance and phagocytosis of mononuclear phagocytes in mice. Meanwhile, MHFP promoted the proliferation of splenic lymphocytes. MHFP could enhance the content of serum hemolysin and improve the decrease of hemolysin induced by cyclophosphamide. The contents of ACP and LDH in the serum and spleen were determined, indicating that MHFP could enhance the activity of macrophages. MHFP promoted the content of cytokines (IL-2, IL-6, TNF-, and IFN-) and mRNA expression. At the same time, the pathological changes of the spleen tissue also showed that MHFP could improve the immunosuppression induced by cyclophosphamide. In addition, MHFP increased the content of SOD, T-AOC, and CAT in the serum and spleen tissue, decreased the level of MDA, and improved the oxidative stress caused by cyclophosphamide. In conclusion, MHFP could effectively improve the immunosuppression and oxidative stress induced by cyclophosphamide and enhance the immune capacity of mice.
本文研究了花多糖（MHFP）的免疫调节作用。通过环磷酰胺建立免疫抑制小鼠模型，用不同剂量的 MHFP（600、400 和 200mg/kg·d）进行处理。结果表明，MHFP 显著增加了脾和胸腺指数，改善了免疫器官的萎缩。MHFP 增强了小鼠单核吞噬细胞的碳廓清能力和吞噬能力。同时，MHFP 促进了脾淋巴细胞的增殖。MHFP 可以提高血清溶血素的含量，改善环磷酰胺引起的溶血素下降。测定血清和脾中 ACP 和 LDH 的含量，表明 MHFP 可以增强巨噬细胞的活性。MHFP 促进了细胞因子（IL-2、IL-6、TNF-α 和 IFN-γ）的含量和 mRNA 表达。同时，脾组织的病理变化也表明，MHFP 可以改善环磷酰胺引起的免疫抑制。此外，MHFP 增加了血清和脾组织中 SOD、T-AOC 和 CAT 的含量，降低了 MDA 的水平，改善了环磷酰胺引起的氧化应激。总之，MHFP 能有效改善环磷酰胺引起的免疫抑制和氧化应激，增强小鼠的免疫能力。
