Department of Otolaryngology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.
Department of Neonatology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.
Mol Med Rep. 2018 Jan;17(1):1269-1274. doi: 10.3892/mmr.2017.7933. Epub 2017 Nov 2.
Recent studies have demonstrated that resveratrol can reduce blood sugar, improve insulin resistance, regulate abnormalities in lipid metabolism, and lower the secretion and expression of inflammatory factors. The present study investigated the anti‑inflammatory effects of resveratrol in animal models of acute pharyngitis, and its possible mechanisms. Commercial ELISA kits were used to measure tumor necrosis factor‑α, interleukin (IL)‑6, macrophage inflammatory protein‑2, cyclooxygenase‑2 levels and caspase‑3/9 activity. Toll‑like receptor (TLR)‑4, myeloid differentiation primary response protein MyD88, phosphorylated (p)‑nuclear factor (NF)‑κB and p‑IκB were analyzed using western blotting. In a rabbit model of acute pharyngitis, it was demonstrated that resveratrol inhibited tumor necrosis factor‑α and interleukin‑6 serum levels, macrophage inflammatory protein‑2 and cyclooxygenase‑2 activity levels, reactive oxygen species production and caspase‑3/9 activity. Resveratrol suppressed NACHT, LRR and PYD domains‑containing protein 3 and caspase‑1 protein expression, and reduced IL‑1β and IL‑18 protein expression in animal models of acute pharyngitis. Additionally, resveratrol suppressed TLR4 and myeloid differentiation primary response protein 88 protein expression, and reduced p‑NF‑κB and increased p‑IκB protein expression in animal models of acute pharyngitis. In conclusion, these findings indicated that the anti‑inflammatory activity of resveratrol prevents acute pharyngitis‑induced inflammation by inhibiting NF‑κB in animal models. Therefore, these data suggested an important clinical application of resveratrol in preventing acute pharyngitis.
最近的研究表明，白藜芦醇可以降低血糖、改善胰岛素抵抗、调节脂代谢异常，并降低炎症因子的分泌和表达。本研究探讨了白藜芦醇在急性咽炎动物模型中的抗炎作用及其可能机制。采用商业 ELISA 试剂盒测定肿瘤坏死因子-α、白细胞介素（IL）-6、巨噬细胞炎症蛋白-2、环氧合酶-2 水平和半胱天冬酶-3/9 活性。采用 Western blot 分析 Toll 样受体（TLR）-4、髓样分化初级反应蛋白 MyD88、磷酸化（p）-核因子（NF）-κB 和 p-IκB。在急性咽炎兔模型中，白藜芦醇抑制肿瘤坏死因子-α和白细胞介素-6 血清水平、巨噬细胞炎症蛋白-2 和环氧合酶-2 活性水平、活性氧产生和半胱天冬酶-3/9 活性。白藜芦醇抑制 NACHT、LRR 和 PYD 结构域包含蛋白 3 和半胱天冬酶-1 蛋白表达，并降低急性咽炎动物模型中白细胞介素-1β和白细胞介素-18 蛋白表达。此外，白藜芦醇抑制 TLR4 和髓样分化初级反应蛋白 88 蛋白表达，增加急性咽炎动物模型中 p-IκB 蛋白表达，降低 p-NF-κB 蛋白表达。综上所述，这些发现表明，白藜芦醇通过抑制 NF-κB 发挥抗炎作用，预防急性咽炎引起的炎症。因此，这些数据提示白藜芦醇在预防急性咽炎方面具有重要的临床应用价值。
