Linnenbach A J, Wojcierowski J, Wu S A, Pyrc J J, Ross A H, Dietzschold B, Speicher D, Koprowski H
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Proc Natl Acad Sci U S A. 1989 Jan;86(1):27-31. doi: 10.1073/pnas.86.1.27.
The monoclonal antibody-defined, tumor-associated antigen GA733 was purified from the SW948 human colorectal carcinoma cell line and its partial amino acid sequence was determined. By using a synthetic oligonucleotide probe, two recombinants were isolated from a total human genomic library. We prove the existence of a family of GA733 genes. One of the genomic isolates is demonstrated to be an intronless gene, which is transcribed in pancreatic carcinoma cell lines and in placenta. The GA733 proteins were observed to contain sequences homologous to a repeat unit occurring 10 times in thyroglobulin and once in the HLA-DR-associated invariant chain. A more evolutionarily distant relationship was found with the alpha chain of the interleukin 2 growth factor receptor.
从SW948人结肠癌细胞系中纯化了单克隆抗体定义的肿瘤相关抗原GA733，并测定了其部分氨基酸序列。利用合成寡核苷酸探针，从人基因组文库中分离出两个重组体。我们证明了GA733基因家族的存在。其中一个基因组分离物被证明是一个无内含子基因，它在胰腺癌细胞系和胎盘中转录。观察到GA733蛋白含有与甲状腺球蛋白中出现10次、HLA-DR相关不变链中出现1次的重复单元同源的序列。发现与白细胞介素2生长因子受体的α链有更远的进化关系。
