Gao Yuqian, Wang Hui, Nicora Carrie D, Shi Tujin, Smith Richard D, Sigdel Tara K, Sarwal Minnie M, Camp David G, Qian Wei-Jun
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
The Department of Surgery, University of California San Francisco, San Francisco, CA, USA.
Methods Mol Biol. 2018;1788:145-156. doi: 10.1007/7651_2017_93.
Liquid chromatography (LC)-selected reaction monitoring (SRM) is a powerful protein quantification technique in terms of sensitivity, reproducibility, and multiplexing capability. LC-SRM can accurately measure the concentrations of surrogate proteotypic peptides for targeted proteins in complex biological samples by using their stable heavy isotope-labeled counterparts as internal standards. Herein, we describe a step-by-step protocol of the application of LC-SRM to quantify candidate protein biomarkers in human urine.
液相色谱（LC）-选择反应监测（SRM）在灵敏度、重现性和多重分析能力方面是一种强大的蛋白质定量技术。LC-SRM可以通过使用稳定的重同位素标记的对应物作为内标，准确测量复杂生物样品中目标蛋白质的替代蛋白型肽段的浓度。在此，我们描述了应用LC-SRM定量人尿液中候选蛋白质生物标志物的详细步骤方案。
