基于液相色谱-串联质谱的尿液蛋白质生物标志物靶向定量分析
LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers.
作者信息
Gao Yuqian, Wang Hui, Nicora Carrie D, Shi Tujin, Smith Richard D, Sigdel Tara K, Sarwal Minnie M, Camp David G, Qian Wei-Jun
机构信息
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
The Department of Surgery, University of California San Francisco, San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2018;1788:145-156. doi: 10.1007/7651_2017_93.
Abstract
Liquid chromatography (LC)-selected reaction monitoring (SRM) is a powerful protein quantification technique in terms of sensitivity, reproducibility, and multiplexing capability. LC-SRM can accurately measure the concentrations of surrogate proteotypic peptides for targeted proteins in complex biological samples by using their stable heavy isotope-labeled counterparts as internal standards. Herein, we describe a step-by-step protocol of the application of LC-SRM to quantify candidate protein biomarkers in human urine.
摘要
液相色谱（LC）-选择反应监测（SRM）在灵敏度、重现性和多重分析能力方面是一种强大的蛋白质定量技术。LC-SRM可以通过使用稳定的重同位素标记的对应物作为内标，准确测量复杂生物样品中目标蛋白质的替代蛋白型肽段的浓度。在此，我们描述了应用LC-SRM定量人尿液中候选蛋白质生物标志物的详细步骤方案。
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