Sheneef Abeer, Mohamed Tamer, Boraey Naglaa F, Mohammed Mostafa A
Department of Medical Microbiology & Immunology, Faculty of Medicine, Sohag University, Sohag, Egypt.
Department of Pediatrics, Faculty of Medicine, Sohag University, Sohag, Egypt.
Egypt J Immunol. 2017 Jan;24(1):29-36.
Neonatal sepsis remains a global health problem particularly in the developing countries. Its diagnosis remains one of the most difficult issues in clinical medicine. Many immunologic markers including neutrophils CD11b and CD 64 and Lipocalin-2 have been tested as biomarkers of neonatal sepsis. The aim of the present study was to assess the value of these markers for early diagnosis of neonatal sepsis. The study included 60 neonates with suspected neonatal sepsis and 20 apparently healthy controls. Lipocalin-2 serum level was assessed by ELISA while neutrophils CD11b and CD64 expressions were evaluated by flow cytometry. Neutrophils CD64 and CD11b expression levels elevated significantly in cases (67.8±7.57) and (57.01±2.46) respectively than in controls (11.78±7.20) and (8.26±4.79). Lipocalin-2 serum level was significantly higher in the patients (145.3 ± 55.3) than in controls (22.4 ± 12.9). In conclusion, neutrophils CD64, CD11b and Lipocalin-2 are early, specific and sensitive diagnostic markers of neonatal sepsis.
新生儿败血症仍然是一个全球性的健康问题，在发展中国家尤为如此。其诊断仍然是临床医学中最困难的问题之一。许多免疫标志物，包括中性粒细胞CD11b、CD64和脂质运载蛋白-2，都已作为新生儿败血症的生物标志物进行了检测。本研究的目的是评估这些标志物在新生儿败血症早期诊断中的价值。该研究纳入了60例疑似新生儿败血症的新生儿和20例明显健康的对照。通过酶联免疫吸附测定法评估脂质运载蛋白-2血清水平，同时通过流式细胞术评估中性粒细胞CD11b和CD64的表达。与对照组（分别为11.78±7.20和8.26±4.79）相比，病例组中性粒细胞CD64和CD11b的表达水平显著升高（分别为67.8±7.57和57.01±2.46）。患者的脂质运载蛋白-2血清水平（145.3±55.3）显著高于对照组（22.4±12.9）。总之，中性粒细胞CD64、CD11b和脂质运载蛋白-2是新生儿败血症早期、特异性和敏感性的诊断标志物。
