Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Jinan 250100, Shandong, China.
Key Laboratory of Plant Cell Engineering and Germplasm Innovation, School of Life Sciences, Shandong University, Jinan 250100, Shandong, China
Plant Physiol. 2018 Jan;176(1):742-756. doi: 10.1104/pp.17.01089. Epub 2017 Nov 9.
NAC proteins constitute one of the largest families of plant-specific transcription factors, and a number of these proteins participate in the regulation of plant development and responses to abiotic stress. (), cloned from the halophyte , is a transcription factor gene, and its overexpression can improve abiotic stress resistance, especially in salt stress tolerance, in both and Arabidopsis () and retard the growth of these plants. In this study, the transcriptional activation activity of TsNAC1 and RD26 from Arabidopsis was compared with the target genes' promoter regions of TsNAC1 from , and the results showed that the transcriptional activation activity of TsNAC1 was higher in tobacco () and yeast. The target sequence of the promoter from the target genes also was identified, and TsNAC1 was shown to target the positive regulators of ion transportation, such as , and the transcription factors and In addition, TsNAC1 negatively regulates the expansion of cells, inhibits and , and directly controls the expression of Based on these results, we propose that TsNAC1 functions as an important upstream regulator of plant abiotic stress responses and vegetative growth.
NAC 蛋白构成了植物特异性转录因子中最大的家族之一，其中一些蛋白参与了植物发育和非生物胁迫响应的调节。（）从盐生植物中克隆而来，是一个 转录因子基因，其过表达可以提高非生物胁迫抗性，特别是在盐胁迫耐受性方面，同时也可以减缓这些植物的生长。在这项研究中，比较了来自拟南芥的 TsNAC1 和 RD26 与来自 的 TsNAC1 目标基因启动子区域的转录激活活性，结果表明 TsNAC1 在烟草（）和酵母中的转录激活活性更高。还鉴定了目标基因启动子的目标序列，表明 TsNAC1 靶向离子转运的正调节剂，如 、转录因子 和 。此外，TsNAC1 负调控细胞的扩张，抑制 和 ，并直接控制 的表达。基于这些结果，我们提出 TsNAC1 作为植物非生物胁迫响应和营养生长的重要上游调节剂发挥作用。
