Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, College of Medicine, Seoul National University, Seoul, Korea.
Sci Rep. 2017 Nov 9;7(1):15230. doi: 10.1038/s41598-017-15458-7.
Recently, we introduced a temperature sensitive Mycobacterium spp., Mycobacterium paragordonae (Mpg). Here, we checked its potential as a candidate for live vaccination against Mycobacterium tuberculosis and Mycobacterium abscessus. Intravenous infections of mice with Mpg led to lower colony forming units (CFUs) compared to infection with BCG, suggesting its usefulness as a live vaccine. The analyses of immune responses indicated that the highly protective immunity elicited by Mpg was dependent on effective dendritic maturation, shift of cytokine patterns and antibody production toward a Th1 phenotype, and enhanced cytotoxic T cell response. Compared to BCG, Mpg showed a more effective protective immune response in the vaccinated mice against challenges with 2 different mycobacterial strains, M. tuberculosis H37Ra or M. abscessus Asan 50594. Our data suggest that a temperature sensitive Mpg may be a potentially powerful candidate vaccine strain to induce enhanced protective immune responses against M. tuberculosis and M. abscessus.
最近，我们介绍了一种温度敏感型分枝杆菌属细菌，即Mycobacterium paragordonae（Mpg）。在这里，我们研究了它作为针对结核分枝杆菌和脓肿分枝杆菌的活疫苗候选物的潜力。与感染卡介苗（BCG）相比，Mpg 静脉感染小鼠后，其菌落形成单位（CFU）更低，这表明它可用作活疫苗。免疫反应分析表明，Mpg 引发的高度保护性免疫依赖于有效的树突状细胞成熟、细胞因子模式的转变以及向 Th1 表型的抗体产生，并增强了细胞毒性 T 细胞反应。与 BCG 相比，Mpg 在接种小鼠中对 2 种不同分枝杆菌菌株（结核分枝杆菌 H37Ra 或脓肿分枝杆菌 Asan 50594）的挑战显示出更有效的保护性免疫反应。我们的数据表明，温度敏感型 Mpg 可能是一种有潜力的强大候选疫苗株，可诱导针对结核分枝杆菌和脓肿分枝杆菌的增强的保护性免疫反应。
