Department of Clinical Laboratory, National Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Infectious Diseases (Tuberculosis), Shenzhen Clinical Research Center for Tuberculosis, The Third People's Hospital of Shenzhen, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Department of Medical Imaging, National Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Infectious Diseases (Tuberculosis), Shenzhen Clinical Research Center for Tuberculosis, The Third People's Hospital of Shenzhen, Southern University of Science and Technology, Shenzhen, People's Republic of China.
Emerg Microbes Infect. 2022 Dec;11(1):1973-1981. doi: 10.1080/22221751.2022.2103453.
(MPG) is an emerging and less common type of Non-tuberculous mycobacteria (NTM) and we know little about its characteristics and prognosis, hence we constructed this retrospective cohort study.
MPG was identified using MALD-TOF MS, multi-target combined gene sequencing and WGS. Clinical information was collected, antimicrobial susceptibility testing was measured using the SLOMYCO panel, and optimal growth temperature testing was measured using Lowenstein-Jensen medium.
Eight MPGs were isolated from 1730 NTMs (0.46%); the mean age of MPG pulmonary disease (MPG-PD) patients was 42.38 ± 9.92 years, 37.5% were male, and the average BMI was 18.4 ± 0.51 kg/m. All patients had the symptoms of cough and sputum and CT images mainly presented in patchy or streaky shadows, MPG grew at 25°C, 30°C and 37°C, and the optimal growth temperature is 37°C. MPGs were sensitive to clarithromycin, rifabutin, amikacin, linezolid, moxifloxacin, cotrimoxazole and ciprofloxacin, two isolates were resistant to rifampicin. Two patients had follow up information, their imaging remained stable during the follow-up.
MPG-PD is a rare NTM disease and is more likely to develop in middle-aged, female, and low BMI patients. The patients present with no specific features within the symptoms as well as the CT imaging. The optimal growth temperature of MPG is at 37°C, MPG-PD has excellent sensitivity to drugs recommended by CLSI and presents with a stable disease.
（MPG）是一种新兴的、不太常见的非结核分枝杆菌（NTM），我们对其特征和预后知之甚少，因此我们进行了这项回顾性队列研究。
采用 MALD-TOF MS、多靶位联合基因测序和 WGS 鉴定 MPG。收集临床资料，采用 SLOMYCO 板进行抗菌药物敏感性试验，采用 Lowenstein-Jensen 培养基进行最佳生长温度试验。
从 1730 株 NTM 中分离出 8 株 MPG（0.46%）；MPG 肺病（MPG-PD）患者的平均年龄为 42.38±9.92 岁，男性占 37.5%，平均 BMI 为 18.4±0.51kg/m。所有患者均有咳嗽、咳痰症状，CT 图像主要表现为斑片状或条纹状阴影，MPG 在 25°C、30°C 和 37°C 下生长，最佳生长温度为 37°C。MPG 对克拉霉素、利福布汀、阿米卡星、利奈唑胺、莫西沙星、复方磺胺甲噁唑和环丙沙星敏感，两株分离株对利福平耐药。有 2 例患者有随访信息，随访期间其影像学保持稳定。
MPG-PD 是一种罕见的 NTM 疾病，更可能发生在中年、女性和低 BMI 患者中。患者的症状和 CT 影像学无特异性表现。MPG 的最佳生长温度为 37°C，MPG-PD 对 CLSI 推荐的药物具有极好的敏感性，疾病稳定。
