Centre for Advanced Materials, Indian Association for the Cultivation of Science , Kolkata 700032, India.
Cellular and Molecular Neuroscience Laboratory, National Brain Research Centre , Manesar, Gurgaon 122051, India.
Langmuir. 2017 Dec 12;33(49):13996-14003. doi: 10.1021/acs.langmuir.7b02202. Epub 2017 Nov 27.
Trehalose is a well-known antiamyloidogenic molecule that inhibits protein aggregation under the intracellular/extracellular condition, and recent work shows that the nanoparticle form of trehalose can further enhance this performance. Here we have designed a trehalose-functionalized Au nanoparticle that can inhibit the aggregation of a polyglutamine-containing mutant protein inside the neuronal cell. Designed nanoparticles have a 20-30 nm Au core with about 350 ± 50 trehalose molecules per particle on the surface on average. They enter the cell, inhibit mutant protein aggregation, and enhance the cell survival against toxic protein aggregates. This work extends the application potential of trehalose for the understanding and treatment of different diseases involving protein aggregation.
海藻糖是一种众所周知的抗淀粉样变性分子，可在细胞内/细胞外条件下抑制蛋白质聚集，最近的研究表明，海藻糖的纳米颗粒形式可以进一步增强这种性能。在这里，我们设计了一种海藻糖功能化的 Au 纳米颗粒，它可以抑制神经元细胞内含有聚谷氨酰胺的突变蛋白的聚集。设计的纳米颗粒具有 20-30nm 的 Au 核，平均每个颗粒表面约有 350±50 个海藻糖分子。它们进入细胞，抑制突变蛋白聚集，并提高细胞对有毒蛋白聚集物的存活率。这项工作扩展了海藻糖在理解和治疗不同蛋白质聚集相关疾病方面的应用潜力。
