Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering , University of Jinan , Jinan , Shandong 250022 , People's Republic of China.
Anal Chem. 2019 May 7;91(9):5513-5516. doi: 10.1021/acs.analchem.9b01457. Epub 2019 Apr 26.
Cysteine (Cys) is an important mediator to regulate the redox state of endoplasmic reticulum (ER), and its level is closely related with many ER stress induced serious diseases. Herein, we present an ER-specific fluorescent probe for the ratiometric imaging of cellular Cys for the first time. The probe exhibited desirable selectivity and sensitivity to Cys. Biological imaging experiments demonstrated that the probe possessed an ER-targeting property, showed ratiometric response to Cys in ER, and could be applied for the ratiometric imaging of Cys level changes during HO-induced redox imbalance in living cells.
半胱氨酸（Cys）是一种重要的介体，可调节内质网（ER）的氧化还原状态，其水平与许多 ER 应激诱导的严重疾病密切相关。本文首次报道了一种用于细胞内 Cys 比率成像的内质网特异性荧光探针。该探针对半胱氨酸具有良好的选择性和灵敏度。生物成像实验表明，该探针具有内质网靶向性，对内质网中的半胱氨酸呈比率响应，可用于 HO 诱导的活细胞氧化还原失衡过程中 Cys 水平变化的比率成像。
