小鼠骨髓来源的髓系抑制细胞的分化及其与脾树突状细胞的共培养。
Differentiation of Myeloid-derived Suppressor Cells from Murine Bone Marrow and Their Co-culture with Splenic Dendritic Cells.
作者信息
Mondanelli Giada, Volpi Claudia
机构信息
Department of Experimental Medicine, Section of Pharmacology, University of Perugia, Perugia, Italy.
出版信息
Bio Protoc. 2017 Sep 20;7(18). doi: 10.21769/BioProtoc.2558.
Abstract
Myeloid-derived suppressor cells (MDSCs) possess the ability to suppress the immune response, and to amplify the regulatory properties of other immune cells, dendritic cells. Here we describe a protocol in which MDSCs were differentiated from murine bone marrow cells, and CD11c dendritic cells were purified from murine spleens. MDSCs and CD11c dendritic cells can be co-cultured and the immunoregulatory phenotype of the MDSCs-conditioned dendritic cells could be assessed by means of a specific functional experiment, a skin test as a measure of the delayed-type hypersensitivity reaction toward a poorly immunogenic antigen.
摘要
髓源性抑制细胞（MDSCs）具有抑制免疫反应以及增强其他免疫细胞（树突状细胞）调节特性的能力。在此，我们描述一种方案，即从鼠骨髓细胞中分化出MDSCs，并从鼠脾脏中纯化出CD11c树突状细胞。MDSCs和CD11c树突状细胞可进行共培养，并且可通过一项特定功能实验（以皮肤试验作为对低免疫原性抗原迟发型超敏反应的一种测量方法）来评估经MDSCs处理的树突状细胞的免疫调节表型。
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