Izhakiella australiensis sp. nov. isolated from an Australian desert soil.

A Gram-stain-negative, rod-shaped, non-motile bacterium, designated D4N98, was isolated from a desert soil near Glendambo, Australia. The taxonomic position of strain D4N98 was investigated using a polyphasic approach. Strain D4N98 shared 97 % 16S rRNA gene sequence similarity with the only reference strain of the genus Izhakiella (Izhakiella capsodis N6PO6), and less than 96 % similarity with other species of genera in the family Enterobacteriaceae with validly published names. Phylogenetic analysis, based on 16S rRNA, rpoB, atpD, gyrB genes and a concatenated sequence comprising 37 single copy marker genes, as well as chemotaxonomic data (major polar lipids: phosphatidyl ethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid, and a glycolipid. Major fatty acids (>10 %): C16 : 0, C17 : 0 cyclo, summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) support the affiliation of this strain to the genus Izhakiella. The results of in silico DNA-DNA hybridisation plus physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain D4N98 from the other species of the genus Izhakiellawith validly published names. Therefore, strain D4N98 represents a novel species, for which the name Izhakiella australiensis sp. nov. is proposed. The type strain is D4N98 (LMG 30066=DSM 105030).