Ji Mukan, Tang Sihui, Ferrari Belinda C
School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Randwick, NSW, 2052, Australia.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4317-4322. doi: 10.1099/ijsem.0.002171.
A Gram-stain-negative, rod-shaped, non-motile bacterium, designated D4N98, was isolated from a desert soil near Glendambo, Australia. The taxonomic position of strain D4N98 was investigated using a polyphasic approach. Strain D4N98 shared 97 % 16S rRNA gene sequence similarity with the only reference strain of the genus Izhakiella (Izhakiella capsodis N6PO6), and less than 96 % similarity with other species of genera in the family Enterobacteriaceae with validly published names. Phylogenetic analysis, based on 16S rRNA, rpoB, atpD, gyrB genes and a concatenated sequence comprising 37 single copy marker genes, as well as chemotaxonomic data (major polar lipids: phosphatidyl ethanolamine, phosphatidylglycerol, diphosphatidylglycerol, a neutral lipid, and a glycolipid. Major fatty acids (>10 %): C16 : 0, C17 : 0 cyclo, summed feature 2 (C14 : 0 3-OH and/or iso-C16 : 1 I), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) support the affiliation of this strain to the genus Izhakiella. The results of in silico DNA-DNA hybridisation plus physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain D4N98 from the other species of the genus Izhakiellawith validly published names. Therefore, strain D4N98 represents a novel species, for which the name Izhakiella australiensis sp. nov. is proposed. The type strain is D4N98 (LMG 30066=DSM 105030).
从澳大利亚格伦丹博附近的沙漠土壤中分离出一株革兰氏染色阴性、杆状、无运动性的细菌，命名为D4N98。采用多相分类法研究了菌株D4N98的分类地位。菌株D4N98与伊扎基埃拉属（Izhakiella capsodis N6PO6）的唯一参考菌株16S rRNA基因序列相似性为97%，与肠杆菌科其他有效发表名称的属的物种相似性低于96%。基于16S rRNA、rpoB、atpD、gyrB基因以及包含37个单拷贝标记基因的串联序列的系统发育分析，以及化学分类数据（主要极性脂质：磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种中性脂质和一种糖脂。主要脂肪酸（>10%）：C16:0、C17:0环丙烷、总和特征2（C14:0 3-OH和/或异-C16:1 I）、总和特征3（C16:1ω7c和/或C16:1ω6c）、总和特征8（C18:1ω7c和/或C18:1ω6c）支持该菌株属于伊扎基埃拉属。电子DNA-DNA杂交以及生理生化测试结果使菌株D4N98与伊扎基埃拉属其他有效发表名称的物种在基因型和表型上得以区分。因此，菌株D4N98代表一个新物种，为此提出名称澳大利亚伊扎基埃拉（Izhakiella australiensis sp. nov.）。模式菌株为D4N98（LMG 30066=DSM 105030）。
