Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94158, USA.
Analyst. 2017 Dec 4;142(24):4618-4622. doi: 10.1039/c7an01695f.
Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.
液滴微流控技术在化学和生物学领域有广泛的应用价值，但它会产生大量的液滴，这些液滴必须进行分析和分类。在这里，我们描述了一种简单的方法，可以使用商业流式细胞仪对大量的双乳液微流控乳液进行分析和分类。我们通过使用它来根据核酸含量来识别液滴来说明该方法。尽管简单，但我们的方法提供了一种通用的方法，可以在没有定制的微流控双乳化剂或分类器的情况下对微流控液滴进行分析和分类。
