Im Joon, Burney Derek P, McDonough Sean P, Nicholson Brigid, Eatroff Adam, Simpson Kenneth W
From the Blue Pearl Veterinary Partners, New York, New York (J.I., A.E.); Veterinary Specialists of North Texas, Dallas, Texas (D.P.B.); College of Veterinary Medicine, Cornell University, Ithaca, New York (S.P.M., K.W.S.); and SAGE Centers for Veterinary Specialty and Emergency Care, Campbell, California (B.N.).
J Am Anim Hosp Assoc. 2018 Jan/Feb;54(1):65-70. doi: 10.5326/JAAHA-MS-6492. Epub 2017 Nov 13.
This case report describes the detection of intrahepatic bacteria in formalin-fixed paraffin-embedded histopathological sections from three dogs with neutrophilic, pyogranulomatous, or lymphoplasmacytic hepatitis and cholangiohepatitis. In each of these cases, eubacterial fluorescence in situ hybridization enabled colocalization of intrahepatic bacteria with neutrophilic and granulomatous inflammation in samples that were negative for bacteria when evaluated by routine hematoxylin and eosin histopathology augmented with histochemical stains. Positive responses to antimicrobial therapy were observed in of 2 out of 2 patients that were treated with antimicrobials. These findings suggest that eubacterial fluorescence in situ hybridization analysis of formalin-fixed paraffin-embedded histopathological sections is more sensitive than conventional histochemical stains for the diagnosis of bacteria-associated canine hepatitis.
本病例报告描述了在三只患有嗜中性、脓性肉芽肿性或淋巴浆细胞性肝炎及胆管肝炎的犬的福尔马林固定石蜡包埋组织病理学切片中检测到肝内细菌。在这些病例中，通过常规苏木精和伊红组织病理学检查并辅以组织化学染色评估时，样本细菌检测呈阴性，但真细菌荧光原位杂交能够使肝内细菌与嗜中性和肉芽肿性炎症共定位。在接受抗菌治疗的2例患者中，2例对抗菌治疗有阳性反应。这些发现表明，福尔马林固定石蜡包埋组织病理学切片的真细菌荧光原位杂交分析在诊断与细菌相关的犬肝炎方面比传统组织化学染色更敏感。
