Song Aiping, Li Peiling, Jiang Jiafu, Chen Sumei, Li Huiyun, Zeng Jun, Shao Yafeng, Zhu Lu, Zhang Zhaohe, Chen Fadi
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2014 Aug 19;15(8):14442-55. doi: 10.3390/ijms150814442.
WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior.
已知WRKY转录因子在许多植物过程中发挥作用。在此，我们对重要观赏植物菊花（Chrysanthemum morifolium）的15个WRKY家族基因进行了表征。共分离出15个不同的序列；最初基于转录组序列扩增内部片段，然后使用RACE（cDNA末端快速扩增）PCR获得全长cDNA。对这15个基因在多种植物激素处理以及生物和非生物胁迫下的转录情况进行了表征。其中一些基因的表现与其与拟南芥WRKY基因的同源性预测一致，但其他一些基因则表现出不同的行为。
