WHO WPRO Regional Reference Measles/Rubella Laboratory and Key Laboratory of Medical Virology Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, No. 155, Changbai Road, Changping District, Beijing, 102206, People's Republic of China.
Beijing Centers for Disease Control and Prevention, No. 16, Hepingli Middle Street, Dongcheng district, Beijing, 100013, People's Republic of China.
Sci Rep. 2017 Nov 13;7(1):15380. doi: 10.1038/s41598-017-15336-2.
Human mastadenovirus species C (HAdV-C) are the most common etiologic agents of respiratory disease in young children and are frequently detected worldwide including China. Two recombinant HAdV-C strains (BJ04 and BJ09) were isolated from infants with acute respiratory infection (ARI) in Beijing in 2012-2013. The whole genome sequences (WGS) of BJ04 and BJ09 were generated and compared to other 35 HAdV-C WGSs publicly available. Phylogenetic analyses showed that the BJ04 strain might be the result of three homologous recombination events involving the parental strains JX173086 (HAdV-1), NC_001405 (HAdV-2) and LC068718 (HAdV-6), whereas BJ09 viral genome might be made of genetic elements from JX173083 (HAdV-1), KF268199 (HAdV-5), and KR699642 (strain CBJ113). Despite intratypic recombination, amino acid analysis showed that the gene repertoire of BJ04 and BJ09 were similar to type 2 viruses. Finally, this analysis revealed that at least three lineages of HAdV-C have been identified in China, represented by BJ04 related to NC_001405, BJ09 related to CBJ113, and KF951595 (strain DD28) related to virus isolated in Japan. This study showed that the frequent recombination played an important driving force for complexity of the HAdV-C epidemic in Beijing, thereby demonstrating the necessity for epidemiological and virological surveillance for HAdV-C in China.
人腺病毒 C 型（HAdV-C）是引起婴幼儿呼吸道疾病的最常见病原体，在全球范围内包括中国都有频繁检出。2012-2013 年，从北京急性呼吸道感染（ARI）婴幼儿中分离到两株重组 HAdV-C 株（BJ04 和 BJ09）。对 BJ04 和 BJ09 的全基因组序列（WGS）进行了生成，并与其他 35 株可公开获得的 HAdV-C WGS 进行了比较。系统进化分析表明，BJ04 株可能是涉及亲本株 JX173086（HAdV-1）、NC_001405（HAdV-2）和 LC068718（HAdV-6）的三次同源重组事件的结果，而 BJ09 病毒基因组可能由 JX173083（HAdV-1）、KF268199（HAdV-5）和 KR699642（株 CBJ113）的遗传元件组成。尽管存在同种型内重组，但氨基酸分析表明 BJ04 和 BJ09 的基因库与 2 型病毒相似。最后，该分析表明，在中国至少已经鉴定出三种 HAdV-C 谱系，以与 NC_001405 相关的 BJ04、与 CBJ113 相关的 BJ09 和 KF951595（株 DD28）为代表，与日本分离的病毒相关。本研究表明，频繁的重组是北京 HAdV-C 流行复杂性的重要驱动力，因此表明有必要在中国进行 HAdV-C 的流行病学和病毒学监测。
