Department of Basic Medical Sciences, Changzhi Medical College, 161 Jiefang East Street, Changzhi, 046000, China.
Center of Hygiene Inspection, Qinghai Center for Disease Control and Prevention, 55 Bayi Middle Road, Xining, 810007, China.
Virol J. 2020 Jul 22;17(1):111. doi: 10.1186/s12985-020-01387-x.
Human adenoviruses (HAdVs) are prevalent in patients with respiratory infections, in which recombination has important implications for viral detection and pathogenicity. However, less HAdVs recombination was reported in Qinghai plateau. In this study, we obtained an HAdV-C strain (QH-1665/2018) isolated from an infant aged one month with influenza-like illness in Qinghai Province in 2018. The whole genome sequence was generated by next-generation sequencing, and compared with that of other HAdV-C strains available in public. The strain QH-1665/2018 genome is comprised of 36,014 nucleotides and encoded 36 putative proteins. Phylogenetic analysis of complete HAdV genomes and 3 major antigen genes (penton, hexon and fiber) showed that strain QH-1665/2018 was clustered into HAdV-1 [P1H1F1]. Recombination analysis based on the RDP4 package and SimPlot software showed that QH-1665/2018 was a recombinant involving HAdV-1, HAdV-2 and HAdV-5, which was then re-confirmed by phylogenetic analysis. Our results suggest that HAdV-C recombination is highly complex, should be focused on, and the epidemiological and virological surveillance should be strengthened in Qinghai Province.
人类腺病毒（HAdV）在呼吸道感染患者中普遍存在，其中重组对病毒检测和致病性具有重要意义。然而，在青藏高原报道的 HAdV 重组较少。在本研究中，我们从 2018 年青海省一名一个月大的患有流感样疾病的婴儿中获得了一株 HAdV-C 株（QH-1665/2018）。通过下一代测序生成了全基因组序列，并与其他公共可用的 HAdV-C 株进行了比较。株 QH-1665/2018 基因组由 36014 个核苷酸组成，编码 36 个假定蛋白。全基因组和 3 个主要抗原基因（五邻体、六邻体和纤维）的系统进化分析表明，株 QH-1665/2018 聚类为 HAdV-1 [P1H1F1]。基于 RDP4 包和 SimPlot 软件的重组分析表明，QH-1665/2018 是一株涉及 HAdV-1、HAdV-2 和 HAdV-5 的重组病毒，随后通过系统进化分析再次得到证实。我们的结果表明，HAdV-C 重组非常复杂，应引起重视，并应加强青海省的流行病学和病毒学监测。
