Department of Pharmaceutical, Chemical and Environmental Sciences, Faculty of Engineering and Science, University of Greenwich, Medway, Central Avenue, Chatham Maritime, Kent, ME4 4TB, UK.
Faculty of Health, Social Care and Education, Anglia Ruskin University, Bishop Hall Lane, Chelmsford, Essex, CM1 1SQ, UK.
Carbohydr Polym. 2017 Sep 15;172:40-48. doi: 10.1016/j.carbpol.2017.05.040. Epub 2017 May 15.
This study aimed to develop advanced biomaterial polysaccharide based dressings to manage pain associated with infected chronic leg ulcers in older adults. Composite carrageenan (CARR) and hyaluronic acid (HA) dressings loaded with lidocaine (LID) and AgNPs were formulated as freeze-dried wafers and functionally characterized for porous microstructure (morphology), mechanical strength, moisture handling properties, swelling, adhesion and LID release. Antimicrobial activity of AgNPs was evaluated (turbidity assay) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus whilst cell viability studies (MTT) was performed on normal adult human primary epidermal keratinocyte cells. The wafers were soft, flexible and elegant in appearance. HA affected the wafer structure by increasing the resistance to compression but still possessed a balance between toughness and flexibility to withstand normal stresses and prevent damage to newly formed skin tissue respectively. Water uptake was influenced by HA, whilst equilibrium water content and LID release were similar for all the formulations, showing controlled release up to 6h. AgNPs loaded CARR/HA wafers were effective in inhibiting the growth of both Gram positive and Gram negative bacteria. MTT assay showed evidence that the AgNPs/LID loaded wafers did not interfere with cell viability and growth. CARR/HA wafers seem to be a promising system to simultaneously deliver LID and AgNPs, directly to infected chronic leg ulcers.
本研究旨在开发先进的基于多糖的生物材料敷料，以管理与老年人感染性慢性腿部溃疡相关的疼痛。载有利多卡因（LID）和 AgNPs 的卡拉胶（CARR）和透明质酸（HA）复合敷料被制成冷冻干燥薄片，并对其多孔微观结构（形貌）、机械强度、水分处理特性、溶胀、粘附和 LID 释放进行功能表征。AgNPs 的抗菌活性（浊度测定）针对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌进行了评估，而正常成人原代表皮角质形成细胞的细胞活力研究（MTT）则进行了评估。薄片柔软、灵活、外观优雅。HA 通过增加抗压缩性来影响薄片结构，但仍具有韧性和弹性之间的平衡，以分别承受正常应力并防止新形成的皮肤组织受损。水摄取受 HA 影响，而所有制剂的平衡水含量和 LID 释放相似，表明控制释放长达 6 小时。载有 AgNPs 的 CARR/HA 薄片有效抑制革兰氏阳性和革兰氏阴性细菌的生长。MTT 试验表明，载有 AgNPs/LID 的薄片不会干扰细胞活力和生长。CARR/HA 薄片似乎是一种很有前途的系统，可以将 LID 和 AgNPs 直接递送到感染性慢性腿部溃疡。
