体外人淋巴细胞中细胞外产生的单线态氧的细胞毒性和遗传毒性作用。

Cytotoxic and genotoxic effects of extracellular generated singlet oxygen in human lymphocytes in vitro.

作者信息

Decuyper-Debergh D, Piette J, Laurent C, van de Vorst A

机构信息

Laboratory of Experimental Physics, University of Liège, Belgium.

出版信息

Mutat Res. 1989 Jan-Feb;225(1-2):11-4. doi: 10.1016/0165-7992(89)90025-0.

DOI:10.1016/0165-7992(89)90025-0

PMID:2913488

Abstract

The induction of sister-chromatid exchanges (SCE) together with the proliferation rate index (PRI) were studied in human lymphocytes in vitro after treatment with singlet oxygen. When produced outside the cells, singlet oxygen can increase the duration of the cellular cycle as measured by an enhancement of the differences between the proliferation rate indexes of the control and the treated cells. A dose-dependent increase in the SCE rate per chromosome was also detected after contact between the singlet oxygen and lymphocytes.

摘要

在用单线态氧处理后，对体外培养的人淋巴细胞中的姐妹染色单体交换（SCE）诱导及增殖率指数（PRI）进行了研究。当在细胞外产生时，通过增强对照细胞与处理细胞增殖率指数之间的差异来衡量，单线态氧可延长细胞周期的持续时间。在单线态氧与淋巴细胞接触后，还检测到每条染色体的SCE率呈剂量依赖性增加。