Center for Biodiversity Research and Biotechnology, Biotec, Federal University of Piauí, Av. São Sebastião, 2819, Reis Veloso, 64202-020 Parnaíba-PI, Brazil.
REQUIMTE/LAQV, Superior Engineering Institute of Porto, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal.
Int J Mol Sci. 2017 Nov 12;18(11):2399. doi: 10.3390/ijms18112399.
Silver nanoparticles have been shown to possess considerable antibacterial activity, but in vivo applications have been limited due to the inherent, but low, toxicity of silver. On the other hand, silver nanoparticles could provide cutaneous protection against infection, due to their ability to liberate silver ions via a slow release mechanism, and their broad-spectrum antimicrobial action. Thus, in this work, we describe the development of a carboxymethyl cellulose-based hydrogel containing silver nanoparticles. The nanoparticles were prepared in the hydrogel in situ, utilizing two variants of cashew gum as a capping agent, and sodium borohydride as the reducing agent. This gum is non-toxic and comes from a renewable natural source. The particles and gel were thoroughly characterized through using rheological measurements, UV-vis spectroscopy, nanoparticles tracking analysis, and transmission electron microscopy analysis (TEM). Antibacterial tests were carried out, confirming antimicrobial action of the silver nanoparticle-loaded gels. Furthermore, rat wound-healing models were used and demonstrated that the gels exhibited improved wound healing when compared to the base hydrogel as a control. Thus, these gels are proposed as excellent candidates for use as wound-healing treatments.
银纳米粒子已被证明具有相当大的抗菌活性，但由于银的固有但低毒性，其体内应用受到限制。另一方面，银纳米粒子可以通过缓慢释放机制释放银离子，并具有广谱抗菌作用，从而为皮肤提供抗感染保护。因此，在这项工作中，我们描述了一种基于羧甲基纤维素的水凝胶中含有银纳米粒子的水凝胶的开发。纳米粒子在水凝胶中原位制备，利用两种腰果胶作为封端剂和硼氢化钠作为还原剂。这种胶是无毒的，来自可再生的天然来源。通过流变测量、紫外可见光谱、纳米颗粒跟踪分析和透射电子显微镜分析（TEM）对颗粒和凝胶进行了全面的表征。进行了抗菌试验，证实了载银纳米粒子凝胶的抗菌作用。此外，还使用大鼠创面愈合模型进行了研究，结果表明，与作为对照的基础水凝胶相比，这些凝胶表现出了更好的创面愈合效果。因此，这些凝胶被提议作为治疗创面愈合的优秀候选材料。
