Zhao Peng, Chen Anjing, Qi Qichao, Zhou Wenjing, Feng Zichao, Wang Jiwei, Yang Ning, Li Xingang, Wang Jian, Huang Qibing, Huang Bin
Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012, China.
Brain Science Research Institute, Shandong University, Jinan 250012, China.
Oncotarget. 2017 Jul 19;8(48):83712-83722. doi: 10.18632/oncotarget.19380. eCollection 2017 Oct 13.
Several single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor A (VEGFA) gene have been previously reported to be associated with glioma susceptibility, but individual studies have demonstrated inconclusive results. In the current study, a meta-analysis was performed to derive a more precise estimation of the involvement of polymorphisms in glioma development. A comprehensive literature search conducted in PubMed, Embase, the Cochrane Library, and OVID databases through February 25, 2017 yielded 4 eligible studies consisting of 2,275 cases and 2,475 controls. Pooled odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were calculated under allele contrast, dominant, recessive, homozygous, and heterozygous models. In general, minor alleles of polymorphisms , , and were associated with increased glioma risk. In contrast, a significant correlation was found between the minor allele of polymorphism and decreased susceptibility to glioma. Moreover, statistically significant associations with glioma risk were observed for polymorphisms and in the meta-analysis although positive associations were not observed in any of the included studies individually. No significant correlations with glioma susceptibility were identified for polymorphisms or except in the recessive model. Finally, stratified analysis on the basis of genotyping method and Hardy-Weinberg equilibrium (HWE) in controls revealed no significant difference between subgroups. Our results indicated that several VEGFA polymorphisms might be risk factors for glioma in Chinese. More studies with larger sample sizes using different ethnicities are needed to provide additional evidence.
先前有报道称，血管内皮生长因子A（VEGFA）基因中的几个单核苷酸多态性（SNP）与胶质瘤易感性相关，但个别研究结果尚无定论。在本研究中，进行了一项荟萃分析，以更精确地评估多态性在胶质瘤发生中的作用。通过在PubMed、Embase、Cochrane图书馆和OVID数据库中进行全面的文献检索，截至2017年2月25日，共获得4项符合条件的研究，包括2275例病例和2475例对照。在等位基因对比、显性、隐性、纯合子和杂合子模型下计算合并比值比（OR）和相应的95%置信区间（CI）。总体而言，多态性、和的次要等位基因与胶质瘤风险增加相关。相比之下，多态性的次要等位基因与胶质瘤易感性降低之间存在显著相关性。此外，在荟萃分析中观察到多态性和与胶质瘤风险存在统计学显著关联，尽管在任何一项纳入研究中均未单独观察到阳性关联。除隐性模型外，未发现多态性或与胶质瘤易感性有显著相关性。最后，根据基因分型方法和对照组的哈迪-温伯格平衡（HWE）进行分层分析，结果显示亚组之间无显著差异。我们的结果表明，在中国人群中，几种VEGFA多态性可能是胶质瘤的危险因素。需要更多使用不同种族、更大样本量的研究来提供更多证据。
