Zhao Liang, Chen Denglong, Yao Qinghua, Li Min
College of Life Sciences and Technology Xinxiang Medical University, Henan.
College of Life Sciences, Fujian Normal University, Fuzhou.
Int J Nanomedicine. 2017 Nov 2;12:8103-8114. doi: 10.2147/IJN.S47256. eCollection 2017.
A series of wound membranes of polyvinyl alcohol and recombinant spider silk protein (pNSR16) was prepared by electrospinning. The membrane was analyzed by scanning electron microscopy, atomic force microscopy, and Fourier transform infrared spectroscopy. The result showed that the three factors that affected average fiber diameter from high to low were, voltage, flow speed, and solidification distance; and the three factors that affected fiber uniformity from high to low were, flow speed, solidification distance, and voltage. The fibers adhered together after being dealt with alcohol. pNSR16 transformed from random coil into β-sheet after being immersed in alcohol. Additionally, the porosity of the electrospun membrane was 84.85%, which was higher than that of cast membrane prepared with the same composition. Experiments of applying electrospun membranes as wound dressing for Sprague Dawley rat wound healing showed that it could promote wound healing and basic fibroblast growth factor expression.
通过静电纺丝制备了一系列聚乙烯醇和重组蜘蛛丝蛋白（pNSR16）的伤口敷料膜。采用扫描电子显微镜、原子力显微镜和傅里叶变换红外光谱对该膜进行了分析。结果表明，对平均纤维直径影响程度由高到低的三个因素依次为电压、流速和凝固距离；对纤维均匀性影响程度由高到低的三个因素依次为流速、凝固距离和电压。用酒精处理后纤维会黏附在一起。pNSR16浸入酒精后由无规卷曲转变为β-折叠结构。此外，静电纺丝膜的孔隙率为84.85%，高于由相同成分制备的流延膜。将静电纺丝膜作为伤口敷料应用于斯普拉格-道利大鼠伤口愈合的实验表明，它可以促进伤口愈合和碱性成纤维细胞生长因子的表达。
