Turdikulova Shahlo, Dalimova Dilbar, Abdurakhimov Abror, Adilov Bekzod, Navruzov Sarimbek, Yusupbekov Abror, Djuraev Mirjalol, Abdujapparov Suleyman, Egamberdiev Dilshod, Mukhamedov Rustam
Institute of Bioorganic Chemistry Academy of Sciences Republic of Uzbekistan.
National Cancer Center of the Ministry of Health of the Republic of Uzbekistan.
Cent Asian J Glob Health. 2016 Dec 13;5(1):227. doi: 10.5195/cajgh.2016.227. eCollection 2016.
Genetic factors play an important role in the development of gastric cancer (GC), a prevalent malignancy in Central Asia. Recent studies have shown that single-nucleotide polymorphisms (SNPs) in several genes are associated with increased GC risk, indicating that genetic variation contributes to gastric carcinogenesis. Located on chromosome 8q24.2, the prostate stem cell antigen (PSCA) gene encodes a 123-amino acid glycoprotein related to the cell-proliferation inhibition and cell-death induction activity. SNPs in PSCA gene have been found to be associated with gastric cancer risk in a genome-wide association study, but results were not conclusive. This study aimed to investigate the association between two polymorphic variants of PSCA gene (rs2294008 and rs9297976) and the susceptibility to gastric cancer in Uzbekistan.
Two hundred sixty eight patients with gastric cancer and a control group of 248 healthy individuals were included in this study. DNA samples isolated from these groups were genotyped using PCR-RFLP method. Comparative analysis of resulting genotypes showed a statistically significant association between CT genotype and gastric cancer (p=0.03, additive model of inheritance, Cochran-Armitage trend test).
Comparative analysis of the distribution of genotypes of rs2976392 polymorphism did not show a statistically significant difference; however, analysis of the distribution of the rs2976392 genotypes in a subgroup of young women revealed a statistically significant (p = 0.04, additive model of inheritance, Cochran-Armitage trend test) increase in the incidence of AA (38%) and AG (56%) genotypes in patients with GC, compared to the controls (20% and 40%).
Our findings support that PSCA rs2294008 and rs9297976 polymorphism may contribute to the susceptibility to gastric cancer. Genotyping of these polymorphisms can potentially be recommended as one of the criteria for identification of high risk groups for gastric cancer development in Uzbekistan.
遗传因素在胃癌（GC）的发生发展中起着重要作用，胃癌是中亚地区一种常见的恶性肿瘤。最近的研究表明，几个基因中的单核苷酸多态性（SNP）与胃癌风险增加有关，这表明遗传变异参与了胃癌的发生过程。前列腺干细胞抗原（PSCA）基因位于8号染色体q24.2上，编码一种123个氨基酸的糖蛋白，与细胞增殖抑制和细胞死亡诱导活性有关。在一项全基因组关联研究中发现，PSCA基因中的SNP与胃癌风险相关，但结果并不确凿。本研究旨在探讨PSCA基因的两个多态性变体（rs2294008和rs9297976）与乌兹别克斯坦人胃癌易感性之间的关系。
本研究纳入了268例胃癌患者和248名健康个体作为对照组。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对从这些组中分离的DNA样本进行基因分型。对所得基因型的比较分析显示，CT基因型与胃癌之间存在统计学显著关联（p = 0.03，遗传加性模型， Cochr an-Armitage趋势检验）。
rs2976392多态性基因型分布的比较分析未显示出统计学显著差异；然而，对年轻女性亚组中rs2976392基因型分布的分析显示，与对照组（20%和（40%）相比，GC患者中AA（38%）和AG（56%）基因型的发生率有统计学显著增加（p = 0.04，遗传加性模型， Cochr an-Armitage趋势检验）。
我们的研究结果支持PSCA rs2294008和rs9297976多态性可能与胃癌易感性有关。这些多态性的基因分型有可能被推荐作为乌兹别克斯坦胃癌发生高危人群识别标准之一。
