Gao Yang, Rao Huijuanzi, Mao Ling-Jing, Ma Qing-Lin
a Marine College , Shandong University , Weihai , P. R. China.
Nat Prod Res. 2019 Feb;33(4):612-615. doi: 10.1080/14786419.2017.1402310. Epub 2017 Nov 17.
The essential oil (EO) from the aerial parts of Leontopodium leontopodioides (Willd.) Beauverd was obtained by hydrodistillation and analysed by GC-FID and GC-MS. Sixty-five compounds were identified which represent 96.2% of the total composition of the EO. The major components of the EO were palmitic acid (11.6%), n-pentadecanal (5.7%), linalool (3.8%), β-ionone (3.3%), hexahydrofarnesyl acetone (3.2%), bisabolone (3.2%) and β-caryophyllene (3.2%). The EO exhibited an excellent antibacterial activity against Staphylococcus aureus and Bacillus subtilis according to the MIC values tested by micro-dilution method. It also exhibited a significant cytotoxicity against HepG2 and MCF-7 cell lines with the IC values of 67.44 and 70.49 μg/mL according to the MTT assay. However, the antioxidant activity test revealed that the EO exhibited a weak DPPH radical-scavenging activity. In conclusion, the EO of L. leontopodioides could be regarded as a bioactive natural product and deserves further study for its potential therapeutic effects.
通过水蒸馏法从火绒草（Leontopodium leontopodioides (Willd.) Beauverd）地上部分提取挥发油（EO），并采用气相色谱 - 火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC - MS）进行分析。共鉴定出65种化合物，占挥发油总成分的96.2%。挥发油的主要成分是棕榈酸（11.6%）、正十五醛（5.7%）、芳樟醇（3.8%）、β - 紫罗兰酮（3.3%）、六氢法呢基丙酮（3.2%）、紫穗槐烯（3.2%）和β - 石竹烯（3.2%）。根据微量稀释法测试的最低抑菌浓度（MIC）值，该挥发油对金黄色葡萄球菌和枯草芽孢杆菌表现出优异的抗菌活性。根据MTT法测定，它对肝癌细胞系HepG2和乳腺癌细胞系MCF - 7也表现出显著的细胞毒性，IC值分别为67.44和70.49 μg/mL。然而，抗氧化活性测试表明该挥发油的二苯基苦味酰基自由基（DPPH）清除活性较弱。总之，火绒草挥发油可被视为一种具有生物活性的天然产物，其潜在治疗作用值得进一步研究。
