Xin Tong, Huang Weijuan, De Riek Jan, Zhang Shuang, Ahmed Selena, Van Huylenbroeck Johan, Long Chunlin
College of Life and Environmental Sciences Minzu University of China Beijing China.
Plant Sciences Unit Institute for Agricultural and Fisheries Research Melle Belgium.
Ecol Evol. 2017 Sep 21;7(21):8915-8926. doi: 10.1002/ece3.3340. eCollection 2017 Nov.
is an arbor tree that has been cultivated in southwestern China by various sociolinguistic groups for esthetic purposes as well as to derive an edible seed oil. This study examined the influence of management, socio-economic factors, and religion on the genetic diversity patterns of utilizing a combination of ethnobotanical and molecular genetic approaches. Semi-structured interviews and key informant interviews were carried out with local communities in China's Yunnan Province. We collected plant material ( = 190 individuals) from five populations at study sites using single-dose AFLP markers in order to access the genetic diversity within and between populations. A total of 387 DNA fragments were produced by four AFLP primer sets. All DNA fragments were found to be polymorphic (100%). A relatively high level of genetic diversity was revealed in samples at both the species ( = 0.3397, = 0.5236) and population (percentage of polymorphic loci = 85.63%, = 0.2937, = 0.4421) levels. Findings further revealed a relatively high degree of genetic diversity within populations (Analysis of Molecular Variance = 96.31%). The higher genetic diversity within populations than among populations of from different geographies is likely due to the cultural and social influences associated with its long cultivation history for esthetic and culinary purposes by diverse sociolinguistic groups. This study highlights the influence of human management, socio-economic factors, and other cultural variables on the genetic and morphological diversity of at a regional level. Findings emphasize the important role of traditional culture on the conservation and utilization of plant genetic diversity.
是一种乔木，中国西南部的不同社会语言群体出于审美目的以及获取可食用的种子油而对其进行栽培。本研究采用民族植物学和分子遗传学方法相结合的方式，考察了管理、社会经济因素和宗教对其遗传多样性模式的影响。在中国云南省的当地社区开展了半结构化访谈和关键 informant 访谈。我们从研究地点的五个种群中收集了植物材料（= 190 个个体），使用单剂量 AFLP 标记来获取种群内和种群间的遗传多样性。四个 AFLP 引物组共产生了 387 个 DNA 片段。所有 DNA 片段均为多态性（100%）。在该物种（= 0.3397，= 0.5236）和种群（多态位点百分比 = 85.63%，= 0.2937，= 0.4421）水平的样本中均揭示出相对较高水平的遗传多样性。研究结果进一步显示该种群内具有相对较高程度的遗传多样性（分子方差分析 = 96.31%）。该物种不同地理种群内的遗传多样性高于种群间，这可能是由于不同社会语言群体出于审美和烹饪目的对其进行长期栽培所带来的文化和社会影响。本研究突出了人类管理、社会经济因素和其他文化变量对该物种区域水平遗传和形态多样性的影响。研究结果强调了传统文化在植物遗传多样性保护和利用方面的重要作用。
