Gavazza A, Rossi G, Lubas G, Cerquetella M, Minamoto Y, Suchodolski J S
School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Department of Veterinary Sciences, University of Pisa, Pisa, Italy.
Vet Comp Oncol. 2018 Mar;16(1):E169-E175. doi: 10.1111/vco.12367. Epub 2017 Nov 20.
Malignant lymphoma B-cell type is the most common canine haematopoietic malignancy. Changes in intestinal microbiota have been implicated in few types of cancer in humans. The aim of this prospective and case-control study was to determine differences in faecal microbiota between healthy control dogs and dogs with multicentric lymphoma. Twelve dogs affected by multicentric, B-cell, stage III-IV lymphoma, and 21 healthy dogs were enrolled in the study. For each dog, faecal samples were analysed by Illumina sequencing of 16S rRNA genes and quantitative PCR (qPCR) for selected bacterial groups. Alpha diversity was significant lower in lymphoma dogs. Principal coordinate analysis plots showed different microbial clustering (P = .001) and linear discriminant analysis effect size revealed 28 differentially abundant bacterial groups in lymphoma and control dogs. The qPCR analysis showed significant lower abundance of Faecalibacterium spp. (q < .001), Fusobacterium spp. (q = .032), and Turicibacter spp. (q = .043) in dogs with lymphoma compared with control dogs. On the contrary, Streptococcus spp. was significantly higher in dogs with lymphoma (q = .041). The dysbiosis index was significantly higher (P < .0001) in dogs with lymphoma. In conclusion, both sequencing and qPCR analyses provided a global overview of faecal microbial communities and showed significant differences in the microbial communities of dogs presenting with multicentric lymphoma compared with healthy control dogs.
恶性淋巴瘤B细胞型是犬类最常见的造血系统恶性肿瘤。肠道微生物群的变化与人类的几种癌症有关。这项前瞻性病例对照研究的目的是确定健康对照犬和多中心淋巴瘤犬粪便微生物群的差异。12只患有多中心B细胞III-IV期淋巴瘤的犬和21只健康犬被纳入研究。对每只犬的粪便样本进行16S rRNA基因的Illumina测序和针对选定细菌类群的定量PCR（qPCR）分析。淋巴瘤犬的α多样性显著降低。主坐标分析图显示了不同的微生物聚类（P = 0.001），线性判别分析效应大小揭示了淋巴瘤犬和对照犬中有28个差异丰富的细菌类群。qPCR分析显示，与对照犬相比，淋巴瘤犬中粪杆菌属（q < 0.001）、梭杆菌属（q = 0.032）和Turicibacter属（q = 0.043）的丰度显著降低。相反，淋巴瘤犬中的链球菌属显著更高（q = 0.041）。淋巴瘤犬的失调指数显著更高（P < 0.0001）。总之，测序和qPCR分析都提供了粪便微生物群落的总体概况，并显示出与健康对照犬相比，患有多中心淋巴瘤的犬的微生物群落存在显著差异。
