College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.
Technology Innovation Research Division, World Institute of Kimchi, Gwangju, South Korea.
J Vet Intern Med. 2023 Mar;37(2):648-659. doi: 10.1111/jvim.16657. Epub 2023 Feb 28.
The association of gut microbiota with cancer etiology and prognosis has been demonstrated in humans and rodents but has not been studied in dogs with different types of tumors.
HYPOTHESIS/OBJECTIVES: To analyze microbiome composition according to tumor progression based on metastasis, recurrence, and therapeutic response in canine tumors.
Thirty-two client-owned dogs were divided into 3 groups: healthy (n = 9), with lymphoma (n = 12), with nonlymphoid tumors (n = 11).
Retrospective case series included animals were divided into subgroups according to the nature and severity of their tumors. Feces were screened for the 16S rRNA gene.
Overall, alpha diversity was significantly reduced in dogs with tumors (n = 23; 12 lymphoid and 11 nonlymphoid) compared to healthy dogs (n = 9). Bacteroides had lower abundance in canine tumors at genus level. Staphylococcus showed significantly reduced abundance in dogs with aggressive tumor progression. Higher white blood cell (WBC) and neutrophil counts and lower hematocrit were significant in dogs with aggressive tumor. Spearman's rank correlation coefficient analysis revealed several measurements that showed moderate to strong correlations, including Coprococcus with total WBC count, neutrophil count, and hematocrit in the aggressive tumor group, and Saccharimonas with serum albumin and sodium concentration in all tumor dogs.
The diversity of the gut microbiome was significantly reduced in dogs with tumors compared to healthy dogs. Correlations were found between changes in blood measurements and changes in microbiome composition in relation to paraneoplastic syndrome.
肠道微生物群与人类和啮齿动物的癌症病因和预后有关，但尚未在具有不同类型肿瘤的犬中进行研究。
假设/目的：根据肿瘤转移、复发和治疗反应，分析犬肿瘤中基于肿瘤进展的微生物组组成。
32 只患犬被分为 3 组：健康（n=9）、淋巴瘤（n=12）、非淋巴瘤肿瘤（n=11）。
回顾性病例系列包括根据肿瘤的性质和严重程度将动物分为亚组。筛选粪便 16S rRNA 基因。
总体而言，与健康犬（n=9）相比，患有肿瘤（n=23；12 例淋巴样肿瘤和 11 例非淋巴样肿瘤）的犬的 alpha 多样性显著降低。在属水平上，犬肿瘤中拟杆菌的丰度较低。在肿瘤进展迅速的犬中，葡萄球菌的丰度显著降低。在肿瘤进展迅速的犬中，白细胞（WBC）和中性粒细胞计数较高，红细胞压积较低。Spearman 秩相关系数分析显示，在肿瘤进展迅速的犬中，Coprococcus 与总 WBC 计数、中性粒细胞计数和红细胞压积呈中度至强相关，Saccharimonas 与所有肿瘤犬的血清白蛋白和钠浓度呈中度至强相关。
与健康犬相比，患有肿瘤的犬的肠道微生物组多样性显著降低。发现血液测量的变化与与副肿瘤综合征相关的微生物组组成变化之间存在相关性。
