Ichiman Y, Yamada T, Yoshida K
Department of Microbiology, St Marianna University School of Medicine, Kawasaki, Japan.
J Appl Bacteriol. 1987 Aug;63(2):165-9. doi: 10.1111/j.1365-2672.1987.tb02699.x.
Passive protective antibodies in 100 samples of normal human sera against challenge with three representative capsular type strains of Staphylococcus epidermidis in mice were examined. Six of them passively protected mice against capsular type I; 17 protected against capsular type II; one against capsular type III; and one against both capsular types I and II. The activities were sensitive to 2-mercaptoethanol and were absorbed out either with rabbit anti-human IgG serum, rabbit anti-human IgA serum or rabbit anti-human IgM serum. Also, the sera activities absorbed out with cell surface polysaccharide extracted from three representative capsular type strains. These results indicate that the protective activities were specifically related to three major immunoglobulins against the above cell surface polysaccharides.
检测了100份正常人血清中的被动保护性抗体，以观察其对小鼠感染三种代表性表皮葡萄球菌荚膜型菌株的保护作用。其中6份血清可被动保护小鼠抵御I型荚膜菌株；17份可抵御II型荚膜菌株；1份可抵御III型荚膜菌株；1份可同时抵御I型和II型荚膜菌株。这些活性对2-巯基乙醇敏感，并且可被兔抗人IgG血清、兔抗人IgA血清或兔抗人IgM血清吸收。此外，血清活性也可被从三种代表性荚膜型菌株中提取的细胞表面多糖吸收。这些结果表明，保护活性与针对上述细胞表面多糖的三种主要免疫球蛋白特异性相关。
