Pharmacology Department, Faculty of Veterinary Medicine, KafrElsheikh University, Kafr El-Sheikh, Egypt.
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, KafrElsheikh University, Kafr El-Sheikh, Egypt.
Parasitol Res. 2020 Jun;119(6):1955-1968. doi: 10.1007/s00436-020-06692-6. Epub 2020 May 13.
Coccidiosis is a crucial parasitic disease of the poultry industry. As a result of the enormous global economic losses and the increased resistance to the conventional anticoccidial agents, there is a continuous need to find new anticoccidials. Here, the anticoccidial effect of the fluoroquinolone lomefloxacin versus diclazuril in experimentally infected broilers was tested for the treatment of Eimeria tenella infection. Ninety 14-day-old Cobb strain broiler chickens were allocated into five groups, each with 18 chicks. Group 1 (G1) was separated as an uninfected negative control and received no treatment; group 2 (G2), infected untreated (positive control); group 3 (G3), infected and treated with lomefloxacin at a dose rate of 100 ppm in drinking water; group 4 (G4), infected and treated with diclazuril at a dose rate of 2.5 ppm in drinking water; group 5 (G5), infected and treated with lomefloxacin at a dose rate of 100 ppm plus diclazuril at dose rate of 2.5 ppm in drinking water. Clinical signs, mortality rates, number of oocysts per gram of faeces (OPG), growth performance parameters (weight gain: WG and feed conversion ratio: FCR), lesion scoring, haematological and serum biochemical analyses, antioxidant biomarkers and histopathologic inspection of the caeca were used as evaluation criteria for the anticoccidial efficacy of both lomefloxacin and diclazuril. The findings herein showed that administration of lomefloxacin and/or diclazuril improved growth performance parameters (WG, FCR) and significantly (P ≤ 0.05) reduced OPG, and diminished the severity of bloody diarrhoea and mortalities. Additionally, haematological indices and serum biochemical parameters such as ALT, AST, ALP, creatinine, uric acid, total proteins, albumin and globulin were improved. Finally, a significant elevation in the levels of the antioxidant biomarkers was observed in the chicks of G3, G4 and G5 as compared with those of G2.
球虫病是家禽业的一种重要寄生虫病。由于全球经济损失巨大，以及常规抗球虫药物的耐药性增加，因此需要不断寻找新的抗球虫药物。本研究旨在测试氟喹诺酮洛美沙星与地克珠利对人工感染柔嫩艾美耳球虫的肉鸡的抗球虫作用。将 90 只 14 日龄科布肉鸡分为 5 组，每组 18 只鸡。第 1 组（G1）为未感染的阴性对照组，不给予任何处理；第 2 组（G2）为感染未处理（阳性对照）组；第 3 组（G3）为感染后用 100 ppm 剂量的洛美沙星饮水治疗组；第 4 组（G4）为感染后用 2.5 ppm 剂量的地克珠利饮水治疗组；第 5 组（G5）为感染后用 100 ppm 剂量的洛美沙星和 2.5 ppm 剂量的地克珠利饮水联合治疗组。临床症状、死亡率、每克粪便中的卵囊数（OPG）、生长性能参数（增重：WG 和饲料转化率：FCR）、病变评分、血液学和血清生化分析、抗氧化生物标志物和盲肠的组织病理学检查，作为评估洛美沙星和地克珠利抗球虫效果的评价标准。结果表明，给予洛美沙星和/或地克珠利可改善生长性能参数（WG、FCR），显著（P≤0.05）降低 OPG，减轻血便和死亡率的严重程度。此外，血液学指标和血清生化参数如 ALT、AST、ALP、肌酐、尿酸、总蛋白、白蛋白和球蛋白也得到改善。最后，与 G2 相比，G3、G4 和 G5 组的抗氧化生物标志物水平显著升高。
