Department of Dermatology and Venereology, Nanfang hospital, Southern Medical University, Guangzhou, 510515, China.
Photodiagnosis Photodyn Ther. 2018 Mar;21:86-90. doi: 10.1016/j.pdpdt.2017.11.005. Epub 2017 Dec 5.
Photodynamic therapy (PDT) has demonstrated good clinical cure rates and low recurrence rates in the treatment of genital warts. Human papillomavirus (HPV) genotypes and viral load assays can reflect the status of persistent or latent infection and serve as a predictor of infection clearance.
Specimens from 41 patients with HPV infection were obtained, and the HPV genotypes and viral load were analyzed using real-time polymerase chain reaction (PCR) assays. Traditional treatment, such as radiofrequency, microwave, or surgical therapy, was used to remove the visible lesions, and then PDT treatment was performed every week. HPV DNA testing was performed at every patient visit and the frequency of PDT treatment was determined by changes in HPV viral loads.
HPV viral loads decreased significantly after PDT treatment. There were significant differences in HPV viral loads between pretherapy and three or six rounds of PDT treatment. Significant differences were also observed between single and multiple type HPV infection after six rounds of PDT treatment. Patients with single type HPV infection had significantly higher rates of negative HPV DNA test results, as compared with patients with multiple infections after six rounds of PDT treatment; however, there was no difference in recurrence rates between the two groups.
Dynamic monitoring of HPV genotypes and viral loads can be used to guide PDT treatment and indicate PDT treatment efficacy in eliminating HPV.
光动力疗法（PDT）在治疗生殖器疣方面显示出良好的临床治愈率和低复发率。人乳头瘤病毒（HPV）基因型和病毒载量检测可反映持续性或潜伏性感染的状态，并可作为清除感染的预测指标。
采集 41 例 HPV 感染患者的标本，采用实时聚合酶链反应（PCR）检测 HPV 基因型和病毒载量。采用传统治疗方法（如射频、微波或手术治疗）去除可见病变，然后每周进行一次 PDT 治疗。每次就诊时进行 HPV DNA 检测，并根据 HPV 病毒载量的变化确定 PDT 治疗的频率。
PDT 治疗后 HPV 病毒载量明显下降。治疗前与 PDT 治疗 3 或 6 轮后 HPV 病毒载量有显著差异。6 轮 PDT 治疗后，单一和多种类型 HPV 感染之间的 HPV 病毒载量也存在显著差异。6 轮 PDT 治疗后，单一型 HPV 感染患者 HPV DNA 检测阴性率明显高于多重感染患者；但两组患者的复发率无差异。
动态监测 HPV 基因型和病毒载量可用于指导 PDT 治疗，并指示 PDT 治疗消除 HPV 的疗效。
