来自印度南部的携带杀白细胞素（PVL）毒素编码噬菌体的分子特征分析
Molecular characterization of Panton-Valentine leukocidin (PVL) toxin-encoding phages from South India.
作者信息
Balaji V, Yamuna D B, Francis Y I, Priya Doss G
机构信息
Department of Clinical Microbiology, Christian Medical College, Vellore, India.
Department of Integrative Biology, School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, India.
出版信息
New Microbes New Infect. 2017 Aug 25;20:34-38. doi: 10.1016/j.nmni.2017.08.005. eCollection 2017 Nov.
Abstract
A total of 19 methicillin-resistant (MRSA) isolates were investigated for Panton-Valentine leukocidin (PVL) toxin, PVL gene sequence variation and PVL-encoding phages. Whole genome sequencing was performed for all isolates. Analysis of MRSA isolates ( = 19) confirmed that most MRSA ( = 11) were positive for the PVL gene and were multidrug resistant. ST772-MRSA-V was the predominant PVL-positive MRSA clone, although all of them were found to carry the ΦIND772PVL phage in the genome. This study provides insights into the evolution of a new lineage of PVL-MRSA and highlights the potential risk of the emergence of multidrug-resistant community-acquired MRSA with high virulence.
摘要
共对19株耐甲氧西林金黄色葡萄球菌（MRSA）分离株进行了杀白细胞素（PVL）毒素、PVL基因序列变异及编码PVL噬菌体的研究。对所有分离株进行了全基因组测序。对MRSA分离株（n = 19）的分析证实，大多数MRSA（n = 11）的PVL基因呈阳性且对多种药物耐药。ST772-MRSA-V是主要的PVL阳性MRSA克隆，尽管发现它们在基因组中均携带ΦIND772PVL噬菌体。本研究为PVL-MRSA新谱系的进化提供了见解，并突出了具有高毒力的多药耐药社区获得性MRSA出现的潜在风险。
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