Schutkowski Alexandra, Hirche Frank, Geissler Stefanie, Radtke Juliane, Stangl Gabriele I
Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany.
J Clin Transl Endocrinol. 2014 Nov 29;2(1):6-13. doi: 10.1016/j.jcte.2014.07.007. eCollection 2015 Mar.
Lupin proteins have repeatedly been shown to exhibit lipid lowering properties and reduce aortic calcification in atherosclerosis models. Despite many efforts on its identification, the component which is responsible for the observed effects is still under debate. Phytic acid which is generally associated with lupin protein isolates has currently been described as bioactive plant compound. The objective of the study was to determine the role of associated phytic acid for the described lupin protein effects. A two-factorial study with ApoE knockout mice was conducted in which mice received lupin protein isolate or casein with or without phytase. Phytic acid was added to the casein diets to a final concentration identical to the lupin protein diets. Here we show that the serum concentrations of cholesterol, lathosterol and desmosterol were lower and the faecal bile acid excretion was higher in the groups fed lupin proteins than in the groups fed casein ( < 0.05). Mice that received the lupin protein diet containing phytic acid were characterized by a lower aortic calcification than mice of the other three groups ( < 0.05). In conclusion, our results show that the cholesterol lowering properties of lupin protein isolate were not caused by phytic acid. However, the hypocalcific action of lupin proteins appears to depend on the combination of lupin proteins and phytic acid.
羽扇豆蛋白在动脉粥样硬化模型中反复被证明具有降低血脂的特性，并能减少主动脉钙化。尽管人们在其鉴定方面付出了诸多努力，但导致这些观察到的效果的成分仍存在争议。通常与羽扇豆蛋白分离物相关的植酸目前被描述为一种生物活性植物化合物。本研究的目的是确定相关植酸在所述羽扇豆蛋白效应中的作用。对载脂蛋白E基因敲除小鼠进行了一项双因素研究，其中小鼠分别接受羽扇豆蛋白分离物或酪蛋白，添加或不添加植酸酶。将植酸添加到酪蛋白饮食中，使其最终浓度与羽扇豆蛋白饮食相同。我们在此表明，喂食羽扇豆蛋白的组中胆固醇、羊毛甾醇和链甾醇的血清浓度较低，粪便胆汁酸排泄量较高，而喂食酪蛋白的组则不然（P<0.05）。接受含植酸的羽扇豆蛋白饮食的小鼠主动脉钙化程度低于其他三组小鼠（P<0.05）。总之，我们的结果表明，羽扇豆蛋白分离物的降胆固醇特性并非由植酸引起。然而，羽扇豆蛋白的低钙化作用似乎取决于羽扇豆蛋白和植酸的组合。
