Ohadi Mandana, Forootanfar Hamid, Rahimi Hamid Reza, Jafari Elham, Shakibaie Mojtaba, Eslaminejad Touba, Dehghannoudeh Gholamreza
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Curr Pharm Biotechnol. 2017;18(11):900-908. doi: 10.2174/1389201018666171122121350.
Recently, the development of a safe bioactive material with antioxidant properties, which can improve healing activity are focusing. Biosurfactants are very famous for their antimicrobial and free radical scavenging activities. Thereof, the main aim of the present study was to investigate the antioxidant and wound healing activity of the lipopeptide biosurfactant (LBS) produced by Acinetobacter junii B6. DPPH radical scavenging activities and FRAP assays were used to measure the antioxidant properties.
For evaluation of the wound healing activity, 36 rats (previously wounded in depilated thoracic region) were randomly distributed into six groups and chromatic, wound contraction, and histopathological feature were examined. The assessment levels of reactive oxygen species (ROS) after LBS exposure were determined using malondialdehyde (MDA), hydrogen peroxide (H2O2), and glutathione (GSH) assay kits.
DPPH assay showed notable scavenging activities at the corresponding concentrations with IC50 value of 0.7 mg/ml. The reductive potency of the LBS showed lower performance at low concentration, while exhibited a remarkable increase at higher concentration. The best histopathological remission was achieved following treatment by 5 mg/ml of the LBS. Scar wounds at day 13 showed the lowest lesion sizes, increased re-epithelialization, hair follicle detection, and decreased amounts of neutrophilic inflammation, immaturity of the wound bed, erythema, edema, capillary, and retention of necrotic tissue.
Results from MDA, H2O2, and GSH levels of the treated sample confirmed the scavenging property of the bacterial derived LBS through ROS. It could be concluded that the pharmaceutical formula encourages the wound healing because of its notable antioxidant capacity.
• DPPH and FRAP assays showed notable scavenging activity. • MDA, H2O2, and GSH; confirmed the scavenging property of the derived biosurfactant through ROS. • Synthesized formula encourages the healing of the wound because of its antioxidant capacity.
近年来，开发具有抗氧化特性且能提高愈合活性的安全生物活性材料成为研究热点。生物表面活性剂以其抗菌和自由基清除活性而闻名。因此，本研究的主要目的是研究琼氏不动杆菌B6产生的脂肽生物表面活性剂（LBS）的抗氧化和伤口愈合活性。采用DPPH自由基清除活性和FRAP分析来测定抗氧化性能。
为评估伤口愈合活性，将36只（先前在脱毛胸部区域受伤）大鼠随机分为六组，检查皮肤颜色、伤口收缩和组织病理学特征。使用丙二醛（MDA）、过氧化氢（H2O2）和谷胱甘肽（GSH）检测试剂盒测定LBS暴露后活性氧（ROS）的评估水平。
DPPH分析显示在相应浓度下具有显著的清除活性，IC50值为0.7 mg/ml。LBS的还原能力在低浓度时表现较低，而在高浓度时显著增加。用5 mg/ml的LBS处理后实现了最佳的组织病理学缓解。第13天的瘢痕伤口显示出最小的损伤尺寸、再上皮化增加、毛囊检测、中性粒细胞炎症数量减少、伤口床不成熟、红斑、水肿、毛细血管和坏死组织残留减少。
处理后样品的MDA、H2O2和GSH水平结果证实了细菌衍生的LBS通过ROS的清除特性。可以得出结论，该药物配方因其显著的抗氧化能力而促进伤口愈合。
• DPPH和FRAP分析显示出显著的清除活性。• MDA、H2O2和GSH证实了衍生生物表面活性剂通过ROS的清除特性。• 合成配方因其抗氧化能力而促进伤口愈合。
