Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Ann Pharm Fr. 2022 May;80(3):280-290. doi: 10.1016/j.pharma.2021.09.005. Epub 2021 Oct 1.
Wound healing is a dynamic process that occurs in the tissue under the skin. During this process, oxidative stress biomarkers are excessively produced, which finally lead to inflammation and cellular damage. In this study, efforts have been made to evaluate the antioxidant effect and wound healing activity topical formulation containing Heliotropium bacciferum Forssk extract. The in vitro antioxidant properties were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities and the ferric reducing antioxidant power (FRAP) assay. The herbal ointments (2.5% w/w and 5% w/w) were prepared from the hydroalcoholic extract of H. bacciferum Forssk and administrated on the induced wounds in Wistar rats. The chromatic assay, percentage of wound contraction, and histopathological studies were used for evaluating the wound healing activity. For the evaluation of reactive oxygen species (ROS), catalase (CAT) activity, superoxide dismutase (SOD), and glutathione (GSH) levels were examined. The DPPH method showed tremendous radical scavenging activities at the corresponding concentrations with EC value of 80μg/mL. Topical application of the ointment (5% w/w) showed the highest wound contraction in comparison to the positive control (treated with CICALFATE™) and the control group (treated with normal saline). Similarly, the histological study of the group treated with the extract ointment (5% w/w) showed full collagen tissue deposition with a complete epidermal regeneration. The results of the assessment of GSH levels as well as CAT and SOD activities in the treated group (5% w/w) confirmed the scavenging property of the extract ointment. Our findings indicated the proper wound healing impact of the topical formulation of H. bacciferum Forssk due to its notable antioxidant capacity.
伤口愈合是一个发生在皮肤下组织的动态过程。在这个过程中，氧化应激生物标志物会过度产生，最终导致炎症和细胞损伤。在这项研究中，我们努力评估含有千里光 Forssk 提取物的局部制剂的抗氧化作用和伤口愈合活性。体外抗氧化性能通过 2,2-二苯基-1-苦基肼（DPPH）清除活性和铁还原抗氧化能力（FRAP）测定来评估。从千里光 Forssk 的水醇提取物中制备草药软膏（2.5%w/w 和 5%w/w），并在 Wistar 大鼠的诱导性伤口上进行给药。使用比色法、伤口收缩百分比和组织病理学研究来评估伤口愈合活性。为了评估活性氧（ROS）、过氧化氢酶（CAT）活性、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）水平。DPPH 法在相应浓度下表现出巨大的自由基清除活性，EC 值为 80μg/mL。与阳性对照（用 CICALFATE™处理）和对照组（用生理盐水处理）相比，软膏（5%w/w）的局部应用显示出最高的伤口收缩率。同样，用提取物软膏（5%w/w）处理的组的组织学研究显示出完整的胶原组织沉积和完全的表皮再生。在治疗组（5%w/w）中 GSH 水平以及 CAT 和 SOD 活性的评估结果证实了提取物软膏的清除特性。我们的研究结果表明，由于其显著的抗氧化能力，千里光 Forssk 的局部制剂具有适当的伤口愈合作用。
