Atalay Fatma, Tatar Arzu, Dincer Büşra, Gündoğdu Betül, Köyceğiz Sinan
Department of Otorhinolaryngology, Atatürk University School of Medicine, Erzurum, Turkey.
Department of Pharmacology, Erzincan Binali Yıldırım University School of Pharmacy, Erzincan, Turkey.
Turk Arch Otorhinolaryngol. 2020 Dec;58(4):241-248. doi: 10.5152/tao.2020.5714. Epub 2020 Dec 1.
This study aimed to explore whether carvacrol (CV) had a protective effect on paclitaxel-induced ototoxicity from biochemical, functional, and histopathological perspectives.
Forty Wistar albino male rats were randomly separated into five groups of eight rats. Group 1 was the control group, so Paclitaxel or CV was not administered. Group 2 was administered i.p. CV at 25 mg/kg once a week; Group 3, was administered i.p. paclitaxel at 5 mg/kg once a week; Group 4 was administered i.p. paclitaxel at 5 mg/kg followed (30 min later) by CV at 25 mg/kg once a week; and Group 5 was administered i.p. CV at 25 mg/kg followed (1 day later) by paclitaxel at 5 mg/kg. once a week. The drugs were administered intraperitoneally once a week for four consecutive weeks, and distortion product otoacoustic emissions (DPOAE) tests were performed at the beginning of the study before the first drug administration and at the end of the study after the last drug administration. All rats were sacrificed, and cochleae were removed for biochemical and histopathological analysis.
Biochemical data indicated that paclitaxel caused oxidative stress in the cochlea. Histopathological findings revealed the loss of outer hair cells in the organ of Corti (CO) and moderate degenerative changes in the stria vascularis (SV). It was observed that DPOAE measurements were significantly reduced at high frequencies. In groups which CV was administered together with paclitaxel, these biochemical, histopathological, and functional changes were favorably reversed.
CV may have a protective effect against paclitaxel-induced ototoxicity when given.
本研究旨在从生化、功能和组织病理学角度探讨香芹酚（CV）对紫杉醇诱导的耳毒性是否具有保护作用。
40只雄性Wistar白化大鼠随机分为五组，每组8只。第1组为对照组，未给予紫杉醇或CV。第2组每周一次腹腔注射25mg/kg的CV；第3组每周一次腹腔注射5mg/kg的紫杉醇；第4组每周一次腹腔注射5mg/kg的紫杉醇（30分钟后），随后每周一次腹腔注射25mg/kg的CV；第5组每周一次腹腔注射25mg/kg的CV（1天后），随后每周一次腹腔注射5mg/kg的紫杉醇。药物每周一次腹腔注射，连续四周，在研究开始时首次给药前和研究结束时最后一次给药后进行畸变产物耳声发射（DPOAE）测试。所有大鼠处死后，取出耳蜗进行生化和组织病理学分析。
生化数据表明紫杉醇在耳蜗中引起氧化应激。组织病理学结果显示柯蒂氏器（CO）中外毛细胞丢失，血管纹（SV）有中度退行性变化。观察到DPOAE测量在高频时显著降低。在CV与紫杉醇联合给药的组中，这些生化、组织病理学和功能变化得到了良好的逆转。
CV给药时可能对紫杉醇诱导的耳毒性具有保护作用。
