Machado Henrique, Tuttle Robert N, Jensen Paul R
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, USA.
Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, USA; Center for Microbiome Innovation, University of California, San Diego, USA.
Curr Opin Microbiol. 2017 Oct;39:136-142. doi: 10.1016/j.mib.2017.10.025. Epub 2017 Nov 23.
Genome sequencing and the application of omic techniques are driving many important advances in the field of microbial natural products research. Despite these gains, there remain aspects of the natural product discovery pipeline where our knowledge remains poor. These include the extent to which biosynthetic gene clusters are transcriptionally active in native microbes, the temporal dynamics of transcription, translation, and natural product assembly, as well as the relationships between small molecule production and detection. Here we touch on a number of these concepts in the context of continuing efforts to unlock the natural product potential revealed in genome sequence data and discuss nomenclatural issues that warrant consideration as the field moves forward.
基因组测序和组学技术的应用正在推动微生物天然产物研究领域取得许多重要进展。尽管取得了这些成果，但天然产物发现流程中仍有一些方面我们了解甚少。这些方面包括生物合成基因簇在天然微生物中的转录活性程度、转录、翻译和天然产物组装的时间动态，以及小分子产生与检测之间的关系。在此，我们结合为挖掘基因组序列数据中揭示的天然产物潜力而持续开展的工作，探讨其中的一些概念，并讨论随着该领域的发展需要考虑的命名问题。
