College of Chemical & Pharmaceutical Engineering, Hebei University of Science & Technology, Shijiazhuang, Hebei, China.
State Key Laboratory Breeding Base, Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science & Technology, Shijiazhuang, Hebei, China.
Prep Biochem Biotechnol. 2020;50(8):763-767. doi: 10.1080/10826068.2020.1734942. Epub 2020 Mar 5.
Vitamin B12 and propionic acid that were simultaneous produced by are both favorable chemicals widely used in food preservatives, medicine, and nutrition. While the carbon source and propionic acid accumulation reflected fermentation efficiency. In this study, using corn stalk as a carbon source and fed-batch fermentation process in an expanded bed adsorption bioreactor was studied for efficient and economic biosynthesis of acid vitamin B12 and propionic. With liquid hot water pretreated corn stalk hydrolysates as carbon source, 28.65 mg L of vitamin B12 and 17.05 g L of propionic acid were attained at 168 h in batch fermentation. In order to optimize the fermentation outcomes, fed-batch fermentation was performed with hydrolyzed corn stalk in expanded bed adsorption bioreactor (EBAB), giving 47.6 mg L vitamin B12 and 91.4 g L of propionic acid at 258 h, which correspond to product yields of 0.37 mg g and 0.75 g g, respectively. The present study provided a promising strategy for economically sustainable production of vitamin B12 and propionic acid by fermentation using biomass cornstalk as carbon source and expanded bed adsorption bioreactor.
同时由产生的维生素 B12 和丙酸都是广泛应用于食品防腐剂、医药和营养领域的有利化学物质。而碳源和丙酸积累则反映了发酵效率。在这项研究中，以玉米秸秆为碳源，在膨胀床吸附生物反应器中采用分批补料发酵工艺，高效、经济地合成了酸维生素 B12 和丙酸。以液体热水预处理的玉米秸秆水解物为碳源，在分批发酵 168 小时后，可获得 28.65 mg/L 的维生素 B12 和 17.05 g/L 的丙酸。为了优化发酵结果，在膨胀床吸附生物反应器 (EBAB) 中采用水解玉米秸秆进行分批补料发酵，在 258 小时时可获得 47.6 mg/L 的维生素 B12 和 91.4 g/L 的丙酸，相应的产物产率分别为 0.37 mg/g 和 0.75 g/g。本研究为利用生物质玉米秸秆作为碳源和膨胀床吸附生物反应器生产维生素 B12 和丙酸提供了一种有前景的经济可持续策略。
