Tadin Antonija, Galic Nada, Marovic Danijela, Gavic Lidia, Klaric Eva, Pejda Slavica, Ugrin Klara, Zeljzic Davor
Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia.
Department of Endodontics and Restorative Dental Medicine, School of Dental Medicine, University of Zagreb, Zagreb, Croatia.
Am J Dent. 2016 Aug;29(4):219-222.
To evaluate the possible geno/cytotoxic effects of dental composite materials by assessing the frequency of micronuclei formation and other nuclear abnormalities in the exfoliated buccal epithelium.
Swabs were taken from the buccal mucosa of 85 young healthy subjects. All participants had healthy dentition or dentition restored only with composite materials. Genotoxicity and cytotoxicity was assessed by micronucleus assay.
The results indicated no significant difference in number of oral mucosa cells with micronuclei in subjects with different numbers of composite restored tooth surfaces (P= 0.476). Also, the number of restored surfaces had no effect on nuclear alterations closely related to cytotoxicity, such as karyolysis (P= 0.572), karyorrehexis (P= 0.573) and picnosis (P= 0.765).
Despite doubts about the safe clinical use of resin composites, this study found no evidence that composite materials trigger long-term cytogenetic damage in the epithelial cells of buccal mucosa in humans. There is no objective and quantifiable evidence of genotoxicity induced by composite restorative materials in clinical practice.
通过评估脱落颊黏膜上皮细胞中微核形成频率及其他核异常情况，来评价牙科复合材料可能的基因/细胞毒性作用。
从85名年轻健康受试者的颊黏膜取材。所有参与者牙齿健康或仅用复合材料修复牙齿。通过微核试验评估基因毒性和细胞毒性。
结果表明，不同数量的复合材料修复牙面的受试者中，有微核的口腔黏膜细胞数量无显著差异（P = 0.476）。此外，修复面数量对与细胞毒性密切相关的核改变，如核溶解（P = 0.572）、核碎裂（P = 0.573）和核固缩（P = 0.765）没有影响。
尽管对树脂复合材料在临床中的安全使用存在疑虑，但本研究未发现证据表明复合材料会引发人类颊黏膜上皮细胞的长期细胞遗传学损伤。在临床实践中，没有客观且可量化的证据表明复合修复材料具有基因毒性。
