Garcia-Graells Cristina, De Keersmaecker Sigrid C J, Vanneste Kevin, Pochet Brigitte, Vermeersch Katie, Roosens Nancy, Dierick Katelijne, Botteldoorn Nadine
1 Service Food-Borne Pathogens, Scientific Institute of Public Health , Brussels, Belgium .
2 Platform Biotechnology and Molecular Biology, Scientific Institute of Public Health , Brussels, Belgium .
Foodborne Pathog Dis. 2018 Feb;15(2):114-117. doi: 10.1089/fpd.2017.2329. Epub 2017 Nov 28.
A collection of 105 colistin-resistant Salmonella isolates collected from 2012 to 2015 in the national surveillance program in Belgium was screened by PCR for the presence of genes mcr-1 and mcr-2. Of these, 1.90% (2/105) and 0.95% (1/105) tested positive for mcr-1 and mcr-2, respectively. The presence of the mcr-1 or mcr-2 determinant has been confirmed by whole genome sequencing and allowed the localization of these two genes on IncX4 type plasmids. We report here the presence of mcr-1 and the first mcr-2 gene in Salmonella ever isolated in the Belgian food chain. Although present at retail since 2012, the occurrence is low and sporadic.
2012年至2015年期间，在比利时国家监测项目中收集的105株耐黏菌素沙门氏菌分离株通过PCR筛选mcr-1和mcr-2基因的存在情况。其中，mcr-1和mcr-2的检测阳性率分别为1.90%（2/105）和0.95%（1/105）。通过全基因组测序证实了mcr-1或mcr-2决定簇的存在，并确定了这两个基因位于IncX4型质粒上。我们在此报告在比利时食物链中首次分离出的沙门氏菌中存在mcr-1和首个mcr-2基因。尽管自2012年起在零售环节就已存在，但发生率较低且呈散发性。
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